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Phospho-Ezrin (Tyr478) Antibody (PA5-104800) in ICC/IF
Immunofluorescent analysis of Phospho-Ezrin (Tyr478) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-Ezrin (Tyr478) polyclonal antibody (Product # PA5-104800) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 59... View More
产品信息
PA5-104800
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human EZR(Accession P15311), corresponding to amino acid residues around phosphorylated Tyr478.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2816273
产品详细信息
Antibody detects endogenous levels of Ezrin only when phosphorylated at Tyrosine 478.
靶标信息
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cytovillin; cytovillin 2; DKFZp762H157; epididymis secretory protein Li 105; EZRI; Ezrin; FLJ26216; MGC1584; p81; villin 2; villin 2 (ezrin); Villin-2
基因别名: AW146364; CVIL; CVL; EZR; HEL-S-105; p81; R75297; VIL2
UniProt ID: (Human) P15311, (Mouse) P26040, (Rat) P31977
Entrez Gene ID: (Human) 7430, (Mouse) 22350, (Rat) 54319
actin binding
protein binding
microtubule binding
protein domain specific binding
protein kinase A catalytic subunit binding
protein kinase A regulatory subunit binding
S100 protein binding
poly(A) RNA binding
cell adhesion molecule binding
actin filament binding
ATPase binding
cadherin binding involved in cell-cell adhesion
cytoskeletal protein binding
protein complex binding
structural molecule activity
actin or actin-binding cytoskeletal protein
negative regulation of transcription from RNA polymerase II promoter
intestinal D-glucose absorption
sphingosine-1-phosphate signaling pathway
cytoskeletal anchoring at plasma membrane
leukocyte cell-cell adhesion
axon guidance
regulation of cell shape
regulation of cell size
positive regulation of gene expression
protein kinase A signaling
gland morphogenesis
membrane to membrane docking
microvillus assembly
astral microtubule organization
actin cytoskeleton reorganization
receptor internalization
regulation of microvillus length
regulation of actin cytoskeleton organization
cellular protein complex localization
positive regulation of multicellular organism growth
cortical microtubule organization
establishment of epithelial cell apical/basal polarity
filopodium assembly
phosphatidylinositol-mediated signaling
positive regulation of protein secretion
negative regulation of T cell receptor signaling pathway
actin filament bundle assembly
establishment of centrosome localization
establishment of endothelial barrier
negative regulation of ERK1 and ERK2 cascade
cellular response to cAMP
protein localization to cell cortex
establishment of protein localization to plasma membrane
positive regulation of establishment of protein localization to plasma membrane
negative regulation of interleukin-2 secretion
regulation of NIK/NF-kappaB signaling
regulation of organelle assembly
terminal web assembly
positive regulation of protein localization to early endosome
positive regulation of cellular protein catabolic process
negative regulation of p38MAPK cascade
positive regulation of early endosome to late endosome transport
establishment or maintenance of apical/basal cell polarity
epithelial cell differentiation
