Invitrogen

Phospho-Histone H4 (Tyr89) Polyclonal Antibody

Phospho-Histone H4 (Tyr89) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-Histone H4 (Tyr89) Antibody (PA5-105105) in IHC (P)

Immunohistochemistry analysis of Phospho-Histone H4 (Tyr89) in human pancreatic cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-Histone H4 (Tyr89) polyclonal antibody (Product # PA5-105105) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-Histone H4 (Tyr89) Antibody in Western Blot (WB)

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产品信息

PA5-105105

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

产品规格

种属反应

Human, Mouse, Pig, Rat, Bovine

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human Histone H4(Accession P62805), corresponding to amino acid residues around phosphorylated Tyr89.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2816578

产品详细信息

Antibody detects endogenous levels of Histone H4 only when phosphorylated at Tyr89.

靶标信息

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: H4 histone family, member A; H4 histone family, member E; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4.1; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4j; histone 2, H4; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; histone cluster 1, H4a; Histone Cluster 4; histone family member; Histone H4; Histone H4A; histone IV, family 2

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基因别名: dJ160A22.2; FO108; H4; H4-12; H4-16; H4-53; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4f16; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; Hist1h4m; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; X04652