Invitrogen

Phospho-IKK alpha/beta (Ser180, Ser181) Polyclonal Antibody

Phospho-IKK alpha/beta (Ser180, Ser181) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-IKK alpha/beta (Ser180, Ser181) Antibody (PA5-104696) in IHC (P)

Immunohistochemistry analysis of Phospho-IKK alpha/beta (Ser180, Ser181) in human normal tissues adjacent to mammary cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-IKK alpha/beta (Ser180, Ser181) polyclonal antibody (Product # PA5-104696) using a dilution of 1:100 (4°C overnight) followed by HRP conjug... View More

Phospho-IKK alpha/beta (Ser180, Ser181) Antibody in Western Blot (WB)

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产品信息

PA5-104696

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human CHUK(Accession O15111), corresponding to amino acid residues around phosphorylated Ser180.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2816169

产品详细信息

Antibody detects endogenous levels of IKK alpha/ beta only when phosphorylated at Serine 180/181.

靶标信息

IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) gene located in chromosome 10 and 8 respectively in humans. Upon stimulation by agents that activate the NF-kB pathway such as TNF alpha, the two subunits of IKK complex, IKK alpha and IKK -beta alongside a regulator NEMO (IKK gamma) phosphorylates IkB proteins. This targets the later for ubiquitin mediated degradation and phosphorylation of NF-kB leading to subsequent translocation to the nucleus. Phosphorylation at serine 176 and 180 located in the activation loop causes a confirmation change that is required for the activation of the kinases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Study on Toll-Like Receptor 2-Mediated Inflammation-Induced Familial Hypertension Combined with Hyperlipemia and Its Mechanism.

Journal of healthcare engineering

Liu J,Li C,Wang Q,Hu H,Li C,Qian J

PA5-104696 was used in Western Blot to investigate the influence of Toll-like receptor 2- (TLR2-) mediated inflammation on the occurrence and development of familial hypertension combined with hyperlipemia and its related mechanism.

Thu Apr 21 00:00:00 EDT 2022

生物信息学

蛋白别名: Conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; I kappa B kinase 1; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; I-kappa-B-kinase beta; IkappaB kinase; IkappaB kinase alpha; IkB kinase alpha subunit; IKK alpha; IKK-1; IKK-2; IKK-A; IKK-a kinase; IKK-B; IKK-beta; IKK2; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of kappaB kinase beta; inhibitor of nuclear factor kappa B kinase beta subunit; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKA; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase beta; Nuclear factor NFkappaB inhibitor kinase alpha; Serine/threonine protein kinase IKBKB; TCF-16; TCF17; Transcription factor 16

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基因别名: AI132552; AI256658; AIM-1; CHUK; Chuk1; Fbx24; Fbxo24; IKBKA; IKBKB; IKK-2; IKK-alpha; IKK-beta; IKK1; IKK2; IKK[b]; IKKA; IKKB; IKKbeta; IMD15; NFKBIKA; NFKBIKB; TCF16