Invitrogen

Phospho-Jak2 (Tyr1007, Tyr1008) Recombinant Rabbit Monoclonal Antibody (JAK2Y10071008-PB6), FITC

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Phospho-Jak2 (Tyr1007, Tyr1008) Antibody in Flow Cytometry (Flow)

Phospho-Jak2 (Tyr1007, Tyr1008) Antibody in Flow Cytometry (Flow)

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Phospho-Jak2 (Tyr1007, Tyr1008) Antibody (MA5-37198) in Flow

Flow cytometry of Phospho-Jak2 (Tyr1007, Tyr1008) using Jurkat cells. Samples were incubated in monoclonal Phospho-Jak2 (Tyr1007, Tyr1008) antibody (Product # MA5-37198). Images correspond with the following conditions: untreated (black), treated with IFNa/IL4/pervanadate (blue).

Phospho-Jak2 (Tyr1007, Tyr1008) Antibody in Flow Cytometry (Flow)

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Phospho-Jak2 (Tyr1007, Tyr1008) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-Jak2 (Tyr1007, Tyr1008) using Jurkat cells. Samples were incubated in monoclonal Phospho-Jak2 (Tyr1007, Tyr1008) antibody (Product # MA5-37198). Images correspond with the following conditions: untreated (black), treated with IFNa/IL4/pervanadate (blue).

产品信息

MA5-37198

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL/1x10^6 cells

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JAK2Y10071008-PB6

抗原

A synthetic phospho-peptide corresponding to residues surrounding Tyr1007/Tyr1008 of human phospho Jak2.

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偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

纯化类型

Protein A/G

保存液

PBS with 0.20% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2897132

靶标信息

Janus kinase 2 (JAK2) is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways and is mutated in polycythemia vera. This kinase contains the catalytic domain (JH1) of JAK2. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activatiors of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes. JAK2 is required for the IFN gamma-receptor complex initiation and JAK1 functions as an amplifier. Some studies have suggested that the role of JAK2 might be performed by Tyk2 and JAK3, if they were positioned correctly within the IFN gamma-receptor complex. Mutations affecting JAK2 can cause Budd-Chiari syndrome, Polycythemia vera, Thrombocythemia 3, myelofibrosis, or Acute myelogenous leukemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: EC 2.7.10.2; JAK 2; JAK-2; Janus kinase 2; Janus kinase 2 (a protein tyrosine kinase); JTK 10; kinase Jak2; Tyrosine-protein kinase JAK2

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基因别名: JAK2; JTK10; THCYT3

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UniProt ID: (Human) O60674

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Entrez Gene ID: (Human) 3717

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protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity receptor binding growth hormone receptor binding interleukin-12 receptor binding protein binding ATP binding protein C-terminus binding protein kinase binding heme binding type 1 angiotensin receptor binding acetylcholine receptor binding histone kinase activity (H3-Y41 specific) SH2 domain binding histone binding phosphatidylinositol 3-kinase binding insulin receptor substrate binding metal ion binding peptide hormone receptor binding

参与通路

MAPK cascade activation of MAPKK activity adaptive immune response protein phosphorylation apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process movement of cell or subcellular component signal transduction enzyme linked receptor protein signaling pathway G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration JAK-STAT cascade tyrosine phosphorylation of STAT protein STAT protein import into nucleus mesoderm development blood coagulation positive regulation of cell proliferation negative regulation of cell proliferation intrinsic apoptotic signaling pathway in response to oxidative stress negative regulation of cardiac muscle cell apoptotic process positive regulation of cell-substrate adhesion positive regulation of phosphatidylinositol 3-kinase signaling cell migration peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway negative regulation of cell-cell adhesion actin filament polymerization cell differentiation erythrocyte differentiation positive regulation of cell migration axon regeneration mineralocorticoid receptor signaling pathway positive regulation of insulin secretion response to lipopolysaccharide positive regulation of phosphoprotein phosphatase activity positive regulation of interleukin-1 beta production positive regulation of tumor necrosis factor production positive regulation of protein import into nucleus, translocation response to hydroperoxide tumor necrosis factor-mediated signaling pathway response to tumor necrosis factor histone H3-Y41 phosphorylation intracellular signal transduction interleukin-12-mediated signaling pathway peptidyl-tyrosine autophosphorylation regulation of cell proliferation tyrosine phosphorylation of Stat3 protein tyrosine phosphorylation of Stat5 protein tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat5 protein activation of JAK2 kinase activity regulation of apoptotic process positive regulation of DNA binding negative regulation of DNA binding negative regulation of neuron apoptotic process positive regulation of GTPase activity innate immune response positive regulation of nitric oxide biosynthetic process positive regulation of cell differentiation negative regulation of heart contraction response to antibiotic protein autophosphorylation platelet-derived growth factor receptor signaling pathway regulation of inflammatory response positive regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation positive regulation of cell activation positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of nitric-oxide synthase biosynthetic process interferon-gamma-mediated signaling pathway regulation of interferon-gamma-mediated signaling pathway growth hormone receptor signaling pathway JAK-STAT cascade involved in growth hormone signaling pathway positive regulation of growth hormone receptor signaling pathway mammary gland epithelium development response to interleukin-12 extrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway