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Invitrogen

Phospho-KCC2 (Ser940) Polyclonal Antibody

3个已发表图片

3 篇参考文献

查看其他27个KCC2抗体

货号 PA5-95678

CNY 6,231.00

100 µL

下单后预计有货时间 15-Apr-2025

批量或定制请求

抗体检测数据 (1)
已发表图片 (3)

Application

Phospho-KCC2 (Ser940) Antibody in Western Blot (WB)

图: 1 / 4

Phospho-KCC2 (Ser940) Antibody (PA5-95678) in WB

Western blot of KCC2 in rat hippocampal lysate showing specific labeling of a band at ~135 kDa corresponding to Phospho-KCC2 (Ser940) polyclonal antibody (Product # PA5-95678) in the first lane (-). Immunolabeling is blocked by pre-adsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).

Phospho-KCC2 (Ser940) Antibody in Western Blot (WB)

Phospho-KCC2 (Ser940) Antibody in Western Blot (WB)

Phospho-KCC2 (Ser940) Antibody in Western Blot (WB)

Phospho-KCC2 (Ser940) Antibody in Western Blot (WB)

Product Image Gallery

Phospho-KCC2 (Ser940) Antibody in Western Blot (WB) — Western blot of KCC2 in rat hippocampal lysate showing specific labeling of a band at ~135 kDa corresponding to Phospho-KCC2 (Ser940) polyclonal antibody (Product # PA5-95678) in the first lane (-). Immunolabeling is blocked by pre-adsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).

Phospho-KCC2 (Ser940) Antibody in Western Blot (WB) — Changes in KCC2 membrane stability and the phosphorylation of KCC2 and NKCC1 in cerebral cortex during the daily cycle. a Top panel shows biotinylated surface fraction and total protein expression of KCC2 in membrane fractions prepared from flash-frozen neocortex at different times of day. For following panels: harvested cerebral cortex lysates were subjected to biotinylated surface fraction and total protein expression of KCC2, or immunoprecipitation (IP) with the indicated KCC2 Thr 1007 phospho-antibody and the immunoprecipitants were then immunoblotted (IB) with the indicated specific KCC2 antibody. Cerebral cortex lysates were also subjected to IB analysis with the indicated total and phospho-specific antibodies. Both KCC2 dimers (D) and KCC2 monomers (M) are indicated. Molecular masses are indicated in kDa on the left-hand side of the Western blots. beta-tubulin immunoblots were used as a loading control in each gel, and a representative example is shown at the bottom. Western blots are from the same quantified samples. b The right panel shows quantification of the results of the Western blots, as assessed by Students' t -test, two-sided ( n = 4, error bars represent the mean +- SEM., four independent experiments). The quantification (ratio calculation) is illustrated in Supplementary Fig. 7 .Source data and uncropped scans are provided as separate sheets in the Source Data file.

Phospho-KCC2 (Ser940) Antibody in Western Blot (WB) — FIGURE 3 Protein expression of KCC2 and NKCC1 in wild-type and Mecp2 - /y hippocampi. Western blots (A) and quantifications of KCC2 (B) , NKCC1 (C) , and the phosphorylated forms of KCC2 (D,E) and NKCC1 (F) in P30-35 and P50-60 WT and Mecp2 - /y hippocampi. n = 3 for both. Mean +- SEM. * P < 0.05, ** P < 0.01, two-tailed unpaired Student's t -test.

Phospho-KCC2 (Ser940) Antibody in Western Blot (WB) — 10.1371/journal.pone.0232967.g003 Fig 3 Quantitative analyses of rn KCC2b and hs NKCC1 phospho-sites upon staurosporine and NEM treatment in HEK rnKCC2b cells. Stably transfected HEK rn KCC2b cells were treated with DMSO (control), 8 muM staurosporine or 0.5 mM NEM, respectively, for 15 min. Cell lysates were harvested and subjected to immunoprecipitation (IP) and immunoblot (IB) with indicated antibodies. D, dimeric KCC2; M, monomeric KCC2. Band intensities were quantified with ImageJ software. ***, p < 0.001; **, p < 0.01; Wilcoxon-Mann-Whitney test (n = 6).

产品信息

PA5-95678

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看3篇已发表文章 3篇已发表文章

免疫沉淀 (IP)

40 µL of antibody per 1mg lysate

-

产品规格

种属反应

Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 conjugated to KLH

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

sequential chromatography

保存液

10mM HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 0.15M NaCl

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2807480

产品详细信息

Antigen Affinity Purified from Pooled Serum

靶标信息

Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Daily rhythm in cortical chloride homeostasis underpins functional changes in visual cortex excitability.

Nature communications

Pracucci E,Graham RT,Alberio L,Nardi G,Cozzolino O,Pillai V,Pasquini G,Saieva L,Walsh D,Landi S,Zhang J,Trevelyan AJ,Ratto GM

Published figure using Phospho-KCC2 (Ser940) polyclonal antibody (Product # PA5-95678) in Western Blot

Sat Nov 04 00:00:00 UTC 2023

Daily rhythm in cortical chloride homeostasis underpins functional changes in visual cortex excitability.

Nature communications

Pracucci E,Graham RT,Alberio L,Nardi G,Cozzolino O,Pillai V,Pasquini G,Saieva L,Walsh D,Landi S,Zhang J,Trevelyan AJ,Ratto GM

Published figure using Phospho-KCC2 (Ser940) polyclonal antibody (Product # PA5-95678) in Western Blot

Sat Nov 04 00:00:00 UTC 2023

The Chloride Homeostasis of CA3 Hippocampal Neurons Is Not Altered in Fully Symptomatic Mepc2-null Mice.

Frontiers in cellular neuroscience

Belaïdouni Y,Diabira D,Zhang J,Graziano JC,Bader F,Montheil A,Menuet C,Wayman GA,Gaiarsa JL

Published figure using Phospho-KCC2 (Ser940) polyclonal antibody (Product # PA5-95678) in Western Blot

Tue May 17 00:00:00 UTC 2022

The Chloride Homeostasis of CA3 Hippocampal Neurons Is Not Altered in Fully Symptomatic Mepc2-null Mice.

Frontiers in cellular neuroscience

Belaïdouni Y,Diabira D,Zhang J,Graziano JC,Bader F,Montheil A,Menuet C,Wayman GA,Gaiarsa JL

Published figure using Phospho-KCC2 (Ser940) polyclonal antibody (Product # PA5-95678) in Western Blot

Tue May 17 00:00:00 UTC 2022

Staurosporine and NEM mainly impair WNK-SPAK/OSR1 mediated phosphorylation of KCC2 and NKCC1.

PloS one

Zhang J,Cordshagen A,Medina I,Nothwang HG,Wisniewski JR,Winklhofer M,Hartmann AM

Published figure using Phospho-KCC2 (Ser940) polyclonal antibody (Product # PA5-95678) in Western Blot

Fri Aug 07 00:00:00 UTC 2020

Staurosporine and NEM mainly impair WNK-SPAK/OSR1 mediated phosphorylation of KCC2 and NKCC1.

PloS one

Zhang J,Cordshagen A,Medina I,Nothwang HG,Wisniewski JR,Winklhofer M,Hartmann AM

Published figure using Phospho-KCC2 (Ser940) polyclonal antibody (Product # PA5-95678) in Western Blot

Fri Aug 07 00:00:00 UTC 2020

生物信息学

蛋白别名: Electroneutral potassium-chloride cotransporter 2; Furosemide-sensitive K-Cl cotransporter; K-Cl cotransporter 2; KCC 2; KCC2a variant 1; KCC2a-S25 variant 1; KCC2b variant 2; mKCC2; Neuronal K-Cl cotransporter; neuronal-specific K-Cl cotransporter; rKCC2; S12A5; Solute carrier family 12 member 5; solute carrier family 12, member 5

基因别名: KCC2; Kiaa1176; mKIAA1176; Slc12a5

UniProt ID: (Mouse) Q91V14, (Rat) Q63633

Entrez Gene ID: (Mouse) 57138, (Rat) 171373

protein binding ammonium transmembrane transporter activity symporter activity cation:chloride symporter activity potassium:chloride symporter activity protein kinase binding secondary carrier transporter

参与通路

transport ion transport potassium ion transport chloride transport chemical synaptic transmission learning organic cation transport ammonium transport multicellular organism growth thermosensory behavior response to drug intracellular pH reduction transmembrane transport ammonium transmembrane transport cation transmembrane transport chloride transmembrane transport

1x1 image pixel for data collection