Invitrogen

Phospho-LRRK2 (Ser935) Recombinant Rabbit Monoclonal Antibody (4J10Z7)

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抗体检测数据 (2)

Phospho-LRRK2 (Ser935) Antibody in Western Blot (WB)

Phospho-LRRK2 (Ser935) Antibody in Western Blot (WB)

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Phospho-LRRK2 (Ser935) Antibody (MA5-55186) in WB

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-LRRK2 (Ser935) Recombinant Monoclonal Antibody (Product # MA5-55186) at 1:1,000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 for 30 minutes after serum-starvation overnight Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking bu... View More

Phospho-LRRK2 (Ser935) Antibody in Western Blot (WB)

Phospho-LRRK2 (Ser935) Antibody in Western Blot (WB)

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Phospho-LRRK2 (Ser935) Antibody in Western Blot (WB) — Western blot analysis of lysates from NIH/3T3 cells, using Phospho-LRRK2 (Ser935) Recombinant Monoclonal Antibody (Product # MA5-55186) at 1:1,000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 for 30 minutes after serum-starvation overnight Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3 min.

Phospho-LRRK2 (Ser935) Antibody in Western Blot (WB) — Western blot analysis of lysates from C6 cells, using Phospho-LRRK2 (Ser935) Recombinant Monoclonal Antibody (Product # MA5-55186) at 1:1,000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 for 30 minutes after serum-starvation overnight Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3 min.

产品信息

MA5-55186

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

4J10Z7

抗原

A synthetic phosphorylated peptide around S935 of human LRRK2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.6 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

产品详细信息

Immunogen Sequence: SNSLG

靶标信息

LRRK2 is a multidomain protein that includes a kinase domain, a GTPase domain, and a leucine-rich repeat (LRR) domain. Mutations in LRRK2 have been associated with Parkinson's disease. LRRK2 is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: BC 300-268; DKFZp434H2111; FLJ45829; Leucine-rich repeat serine/threonine-protein kinase 2; LRR kinase 2; LRRK 2; LRRK-2

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基因别名: 4921513O20Rik; 9330188B09Rik; AW561911; cI-46; D630001M17Rik; Gm927; Lrrk2

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UniProt ID: (Mouse) Q5S006

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Entrez Gene ID: (Mouse) 66725, (Rat) 300160

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SNARE binding nucleotide binding glycoprotein binding actin binding GTPase activity protein kinase activity protein serine/threonine kinase activity MAP kinase kinase activity GTPase activator activity protein binding ATP binding GTP binding tubulin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Rho GTPase binding syntaxin-1 binding clathrin binding GTP-dependent protein kinase activity peroxidase inhibitor activity identical protein binding protein homodimerization activity ion channel binding protein kinase A binding molecular_function scaffold/adaptor protein

参与通路

MAPK cascade activation of MAPKK activity activation of MAPK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation protein phosphorylation endocytosis autophagy response to oxidative stress mitochondrion organization Golgi organization small GTPase mediated signal transduction neuromuscular junction development determination of adult lifespan positive regulation of autophagy regulation of protein kinase A signaling negative regulation of protein processing regulation of neuron maturation phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation olfactory bulb development tangential migration from the subventricular zone to the olfactory bulb cell differentiation positive regulation of protein ubiquitination negative regulation of protein binding positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process negative regulation of GTPase activity cellular response to oxidative stress cellular protein localization intracellular signal transduction regulation of kidney size exploration behavior locomotory exploration behavior synaptic vesicle recycling regulation of locomotion regulation of membrane potential positive regulation of programmed cell death positive regulation of GTPase activity GTP metabolic process protein autophosphorylation intracellular distribution of mitochondria neuron projection morphogenesis mitochondrion localization regulation of mitochondrial depolarization regulation of synaptic transmission, glutamatergic excitatory postsynaptic potential regulation of dopamine receptor signaling pathway positive regulation of dopamine receptor signaling pathway regulation of dendritic spine morphogenesis neuron death cellular response to manganese ion reactive oxygen species metabolic process regulation of neuron death negative regulation of neuron death negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of protein autoubiquitination regulation of neuroblast proliferation regulation of synaptic vesicle transport negative regulation of thioredoxin peroxidase activity by peptidyl-threonine phosphorylation negative regulation of hydrogen peroxide-induced cell death negative regulation of protein targeting to mitochondrion cellular response to dopamine regulation of branching morphogenesis of a nerve regulation of synaptic vesicle exocytosis negative regulation of peroxidase activity cellular response to organic cyclic compound