Invitrogen

Phospho-Lyn (Tyr396) Recombinant Rabbit Monoclonal Antibody (ARC1597)

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Phospho-Lyn (Tyr396) Antibody in Western Blot (WB)

Phospho-Lyn (Tyr396) Antibody in Western Blot (WB)

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Phospho-Lyn (Tyr396) Antibody (MA5-35882) in WB

Western blot analysis of Phospho-Lyn (Tyr396) in extracts of NIH/3T3 cells. Samples were incubated with Phospho-Lyn (Tyr396) Monoclonal antibody (Product # MA5-35882) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 cells were treated by pervanadate (1 mM) at 37°C for 30 minutes after serum-starvation overnight. Lysates/proteins: 2... View More

Phospho-Lyn (Tyr396) Antibody in Western Blot (WB)

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Phospho-Lyn (Tyr396) Antibody in Western Blot (WB) — Western blot analysis of Phospho-Lyn (Tyr396) in extracts of NIH/3T3 cells. Samples were incubated with Phospho-Lyn (Tyr396) Monoclonal antibody (Product # MA5-35882) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 cells were treated by pervanadate (1 mM) at 37°C for 30 minutes after serum-starvation overnight. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

产品信息

MA5-35882

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

ARC1597

抗原

A synthetic phosphorylated peptide around Y396 of human Lyn (P07948)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2849780

产品详细信息

Immunogen sequence: DNEYT

靶标信息

Lyn proto oncogene (Lcl/Yes related novel protein tyrosine kinase) is encoded by the Lyn gene located on chromosome 8 in humans and belongs to the Src family kinase. It is a non-receptor tyrosine kinase that acts as a mediator in transducing cellular signaling. The most well studied expression of Lyn is in hematopoietic cell types, both lymphoid and myeloid cells with exclusion in T lymphocytes. It has also been observed that Lyn plays diverse roles such as in regulation of B cell receptor signaling, mast cell signaling and Epithelial - Mesenchymal Transition (EMT).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: FLJ26625; Lck/Yes-related novel protein tyrosine kinase; lyn protein non-receptor kinase; p53Lyn; p56Lyn; Tyrosine-protein kinase Lyn; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v-yes-1) oncogene homolog

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基因别名: AA407514; Hck-2; JTK8; LYN; p53Lyn; p56Lyn

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UniProt ID: (Human) P07948, (Mouse) P25911, (Rat) Q07014

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Entrez Gene ID: (Human) 4067, (Mouse) 17096, (Rat) 81515

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protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity receptor binding platelet-derived growth factor receptor binding integrin binding protein binding ATP binding SH3 domain binding ubiquitin protein ligase binding gamma-tubulin binding glycosphingolipid binding ion channel binding phosphoprotein binding nucleotide binding protein kinase activity erythropoietin receptor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups enzyme binding protein complex binding

参与通路

B cell homeostasis regulation of cytokine production negative regulation of protein phosphorylation immune system process Fc receptor mediated stimulatory signaling pathway tolerance induction to self antigen histamine secretion by mast cell platelet degranulation negative regulation of myeloid leukocyte differentiation immune response-regulating cell surface receptor signaling pathway Fc receptor mediated inhibitory signaling pathway regulation of B cell apoptotic process protein phosphorylation inflammatory response cellular response to DNA damage stimulus signal transduction transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation response to hormone positive regulation of neuron projection development phosphorylation peptidyl-tyrosine phosphorylation hemopoiesis erythrocyte differentiation positive regulation of cell migration negative regulation of B cell proliferation neuron projection development lipopolysaccharide-mediated signaling pathway regulation of mast cell activation regulation of cell adhesion mediated by integrin negative regulation of toll-like receptor 2 signaling pathway negative regulation of toll-like receptor 4 signaling pathway intracellular signal transduction peptidyl-tyrosine autophosphorylation positive regulation of phosphorylation positive regulation of tyrosine phosphorylation of STAT protein hemoglobin biosynthetic process regulation of mast cell degranulation negative regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity innate immune response regulation of erythrocyte differentiation protein autophosphorylation cytokine secretion regulation of cytokine secretion regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation B cell receptor signaling pathway regulation of B cell receptor signaling pathway positive regulation of B cell receptor signaling pathway positive regulation of cellular component movement regulation of release of sequestered calcium ion into cytosol positive regulation of glial cell proliferation positive regulation of Fc receptor mediated stimulatory signaling pathway positive regulation of stress-activated protein kinase signaling cascade regulation of ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of oligodendrocyte progenitor proliferation negative regulation of mast cell proliferation positive regulation of mast cell proliferation cellular response to retinoic acid cellular response to peptide hormone stimulus regulation of monocyte chemotaxis regulation of platelet aggregation dendritic cell differentiation negative regulation of intracellular signal transduction positive regulation of dendritic cell apoptotic process regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway adaptive immune response response to sterol depletion central nervous system development blood coagulation response to toxic substance response to carbohydrate oligodendrocyte development response to organic cyclic compound viral process signal transduction by protein phosphorylation platelet activation T cell costimulation cellular response to extracellular stimulus response to insulin cellular response to heat Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis response to drug response to amino acid ephrin receptor signaling pathway response to axon injury negative regulation of immune response leukocyte migration JAK-STAT cascade involved in growth hormone signaling pathway chemotaxis cell migration response to peptide hormone