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Application
FIGURE: 1 / 1
Phospho-MAPRE3 (Ser176) Antibody (PA5-143685) in WB
Western blot of Phospho-MAPRE3 (Ser176) in adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (lanes 1 & 2), and rabbit anti-EB3 (Ser162) polyclonal (lanes 3 & 4), anti-Phospho-MAPRE3 (Ser176) polyclonal antibody (Product # PA5-143685) (lanes 5 & 6), and anti-EB3 polyclonal (a.a. 171-182) (lanes 7 & 8).
Product Details
PA5-143685
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-EB3 (Ser-176) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 176 in human EB3. This peptide sequence is highly conserved in mouse and rat EB3. This site is conserved in EB2 but the neighboring amino acids are not well conserved.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942913
Product Specific Information
This antibody was cross-absorbed to unphospho-EB3 (Ser-176), before affinity purification using phospho-EB3 (Ser-176) peptide (without carrier). The antibody detects a 32 kDa* protein corresponding to the apparent molecular mass of phosphorylated EB3 on SDS-PAGE immunoblots of mouse brain. This reactivity is significantly reduced after lambda phosphatase treatment.
Target Information
EB3 is a 282 amino acid homologue of EB1, a protein known to bind APC (adenomatous polyposis coli). It has been shown to bind to tubulin in vitro and microtubules in vivo. Northern blotting shows expressions to be mainly in brain, and to a lesser degree in muscle. EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APC binding protein; EB1 protein family member 3; EB3; EBF3; End-binding protein 3; Microtubule-associated protein RP/EB family member 3; microtubule-associated protein, RP/EB family, member 3; RP3
Gene Aliases: AI790651; EB2; EB3; EBF3; EBF3-S; MAPRE3; RP3
UniProt ID: (Human) Q9UPY8, (Mouse) Q6PER3, (Rat) Q5XIT1
Entrez Gene ID: (Human) 22924, (Mouse) 100732, (Rat) 298848
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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