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Invitrogen
Phospho-MEK1 (Thr386) Polyclonal Antibody
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
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Phospho-MEK1 (Thr386) Antibody (PA1-4631) in ICC/IF
Immunofluorescence analysis of MEK1 (Thr386) was performed using 90% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-MEK1 (Thr386) Rabbit Polyclonal Antibody (Product # PA1-... View More
产品信息
PA1-4631
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 of human MAP2K1 conjugated to KLH
偶联物
形式
Liquid
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_560048
产品详细信息
This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primate and Xenopus based on 100% sequence homology.
This antibody is specific for the ~45 kDa MEK 1 protein phosphorylated at Thr386 in Western blots of human brain extracts.
靶标信息
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx. Mek1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is known to be involved in the signaling during stress activate response, apoptosis and proliferative induction by cytokines. Mutations in the gene can lead to cardiofaciocutaneous syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Dual specificity mitogen-activated protein kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; kinase MEK1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; mitogen-activated protein kinase kinase 1; MKK (Thr292); MKK (Thr386); MKK2; MP2K1; PRKMK2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
基因别名: CFC3; MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1
UniProt ID: (Human) Q02750, (Rat) Q01986
Entrez Gene ID: (Human) 5604, (Rat) 170851
protein kinase activity
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase activity
protein serine/threonine/tyrosine kinase activity
protein tyrosine kinase activity
receptor signaling protein tyrosine phosphatase activity
protein binding
ATP binding
protein C-terminus binding
protein kinase binding
protein serine/threonine kinase activator activity
protein N-terminus binding
Ras GTPase binding
mitogen-activated protein kinase kinase kinase binding
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPK activity
movement of cell or subcellular component
chemotaxis
cell cycle arrest
signal transduction
heart development
negative regulation of cell proliferation
positive regulation of gene expression
negative regulation of gene expression
peptidyl-tyrosine phosphorylation
cerebellar cortex formation
neuron differentiation
keratinocyte differentiation
thyroid gland development
regulation of stress-activated MAPK cascade
proteolysis in other organism
positive regulation of transcription, DNA-templated
thymus development
regulation of axon regeneration
cell motility
positive regulation of axonogenesis
Bergmann glial cell differentiation
face development
trachea formation
epithelial cell proliferation involved in lung morphogenesis
placenta blood vessel development
labyrinthine layer development
ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
positive regulation of protein serine/threonine kinase activity
regulation of Golgi inheritance
cellular senescence
mitophagy in response to mitochondrial depolarization
positive regulation of production of miRNAs involved in gene silencing by miRNA
regulation of early endosome to late endosome transport
regulation of vascular smooth muscle contraction
protein phosphorylation
response to oxidative stress
mitotic nuclear division
cell proliferation
positive regulation of cell migration
melanosome transport
positive regulation of transcription elongation from RNA polymerase II promoter
negative regulation of homotypic cell-cell adhesion
peptidyl-tyrosine dephosphorylation
intracellular signal transduction
positive regulation of GTPase activity
positive regulation of cell differentiation
positive regulation of Ras protein signal transduction
vesicle transport along microtubule
Golgi inheritance
response to axon injury
neuron projection morphogenesis
protein heterooligomerization
response to glucocorticoid
lung morphogenesis
