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Phospho-NuMA (Thr2055) Antibody (PA5-104623) in ICC/IF
Immunocytochemistry analysis of Phospho-NuMA (Thr2055) in Heat-shock treated Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-NuMA (Thr2055) polyclonal antibody (Product # PA5-104623) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG... View More
产品信息
PA5-104623
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human NUMA1(Accession Q14980), corresponding to amino acid residues around phosphorylated Thr2055.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2816098
产品详细信息
Antibody detects endogenous levels of NuMA only when phosphorylated at Thr2055.
靶标信息
NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: centrophilin stabilizes mitotic spindle in mitotic cells; NMP-22; Nuclear matrix protein-22; Nuclear mitotic apparatus protein; Nuclear mitotic apparatus protein 1; NuMA protein; SP-H antigen; structural nuclear protein
基因别名: 6720401E04Rik; AA764025; AL022610; AU014979; NMP-22; NMP22; NUMA; NUMA1
UniProt ID: (Human) Q14980, (Mouse) E9Q7G0
Entrez Gene ID: (Human) 4926, (Rat) 308870, (Mouse) 101706
