Invitrogen

Phospho-PAK1/PAK2 (Thr423, Thr402) Polyclonal Antibody

查看其他4个PAK1/PAK2抗体
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说明书
实验方案
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产品信息

PA5-105202

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:2,000
-

免疫组化（石蜡） (IHC (P))

1:50-1:200
-
产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human PAK1(Accession Q13153), corresponding to amino acid residues around phosphorylated Thr423.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2816675

产品详细信息

Antibody detects endogenous levels of PAK1 /PAK2 only when phosphorylated at Thr423.

靶标信息

Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: activated protein kinase alpha; Alpha-PAK; CDC42/RAC effector kinase PAK-A; gamma pak; Gamma-PAK; OPHN3; p21 (CDKN1A)-activated kinase 1; p21 (CDKN1A)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 1; p21-activated kinase 2; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p58; p65-PAK; p68-PAK; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK3; PAK65; Protein kinase MUK2; S6/H4 kinase; Serine/threonine-protein kinase PAK 1; Serine/threonine-protein kinase PAK 2; STE20 homolog, yeast

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基因别名: 5330420P17Rik; A130002K10Rik; AI836325; AW045634; D16Ertd269e; gamma-PAK; mKIAA4182; PAK-1; PAK-2; PAK1; PAK2; PAK65; Paka; PAKalpha; PAKgamma

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UniProt ID: (Human) Q13153, (Human) Q13177, (Rat) P35465, (Mouse) Q8CIN4, (Rat) Q64303

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Entrez Gene ID: (Human) 5058, (Human) 5062, (Mouse) 18479, (Rat) 29431, (Mouse) 224105, (Rat) 29432

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功能
protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity protein binding collagen binding ATP binding protein kinase binding protein tyrosine kinase activator activity identical protein binding cadherin binding involved in cell-cell adhesion nucleotide binding catalytic activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Rac GTPase binding MAP kinase kinase kinase activity non-receptor serine/threonine protein kinase
参与通路
MAPK cascade mitotic cell cycle response to hypoxia positive regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway protein phosphorylation exocytosis apoptotic process axonogenesis positive regulation of cell proliferation positive regulation of cell migration T cell costimulation actin cytoskeleton reorganization cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation positive regulation of intracellular estrogen receptor signaling pathway Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis wound healing positive regulation of JUN kinase activity protein autophosphorylation ephrin receptor signaling pathway branching morphogenesis of an epithelial tube neuron projection morphogenesis T cell receptor signaling pathway positive regulation of stress fiber assembly negative regulation of cell proliferation involved in contact inhibition negative regulation of protein kinase activity signal transduction viral process phosphorylation peptidyl-serine phosphorylation signal transduction by protein phosphorylation regulation of growth negative regulation of apoptotic process vascular endothelial growth factor receptor signaling pathway regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation dendritic spine development positive regulation of protein tyrosine kinase activity cellular response to organic cyclic compound cell-cell adhesion positive regulation of extrinsic apoptotic signaling pathway negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis Rho protein signal transduction regulation of mitotic cell cycle neuromuscular junction development metabolic process response to organic substance regulation of gene expression dendrite development cell migration amygdala development actin cytoskeleton organization stress-activated protein kinase signaling cascade activation of protein kinase activity regulation of actin cytoskeleton organization regulation of apoptotic process receptor clustering regulation of MAPK cascade gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission observational learning regulation of long-term synaptic potentiation activation of MAPKK activity small GTPase mediated signal transduction intracellular signal transduction
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