Invitrogen

Phospho-PDPK1 (Tyr376) Polyclonal Antibody

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产品信息

PA5-105859

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000-1:3,000
-

免疫组化（石蜡） (IHC (P))

1:50-1:200
-
产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human PDPK1(Accession O15530), corresponding to amino acid residues around phosphorylated Tyr376.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2817258

产品详细信息

Antibody detects endogenous levels of PDPK1 only when phosphorylated at Tyr376.

靶标信息

PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; HGNC:8809; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; mPDK1; OTTHUMP00000174525; OTTHUMP00000205076; PkB kinase; PkB kinase like gene 1; PkB-like 1; Protein kinase B kinase

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基因别名: PDK1; PDPK1; PDPK2; PDPK2P; PRO0461

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UniProt ID: (Human) Q6FI20, (Mouse) Q9Z2A0, (Rat) O55173

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Entrez Gene ID: (Human) 5170, (Mouse) 18607, (Rat) 81745

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功能
protein serine/threonine kinase activity 3-phosphoinositide-dependent protein kinase activity insulin receptor binding protein binding ATP binding phospholipase activator activity kinase activity protein kinase binding phospholipase binding nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
参与通路
stimulatory C-type lectin receptor signaling pathway type B pancreatic cell development transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation negative regulation of protein kinase activity hyperosmotic response epidermal growth factor receptor signaling pathway positive regulation of phospholipase activity regulation of endothelial cell migration negative regulation of cardiac muscle cell apoptotic process cell migration peptidyl-serine phosphorylation peptidyl-threonine phosphorylation calcium-mediated signaling actin cytoskeleton organization platelet activation negative regulation of transforming growth factor beta receptor signaling pathway T cell costimulation activation of protein kinase B activity cellular response to insulin stimulus negative regulation of toll-like receptor signaling pathway intracellular signal transduction Fc-epsilon receptor signaling pathway regulation of I-kappaB kinase/NF-kappaB signaling regulation of mast cell degranulation protein autophosphorylation phosphatidylinositol-mediated signaling focal adhesion assembly T cell receptor signaling pathway positive regulation of release of sequestered calcium ion into cytosol cellular response to epidermal growth factor stimulus positive regulation of establishment of protein localization to plasma membrane extrinsic apoptotic signaling pathway signal transduction phosphorylation
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