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Invitrogen
Phospho-PKC lambda/iota (Thr555, Thr563) Recombinant Rabbit Monoclonal Antibody (31H34L29)
Application
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Phospho-PKC lambda/iota (Thr555, Thr563) Antibody (700582) in IHC (P)
Immunohistochemistry analysis of PRKC1 (PT555)/ (PT563) showing staining in the cytoplasm of paraffin-embedded mouse skeletal muscle (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at r... View More
产品信息
700582
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
31H34L29
抗原
proprietary
偶联物
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532331
产品详细信息
This antibody is predicted to react with mouse, rat, primate, canine, chicken, equine, bovine, porcine, zebrafish and Xenopus based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
The PKC family of serine/threonine kinases, including PRKCI (PKC iota), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: aPKC-lambda/iota; Atypical protein kinase C-lambda/iota; nPKC-iota; PRKC-lambda/iota; Protein kinase C iota type; protein kinase C, iota; protein kinase C, lambda
基因别名: 2310021H13Rik; AI427505; aPKClambda; DXS1179E; mKIAA4165; nPKC-iota; PKCI; Pkcl; PKClambda; PRKCI; Prkcl
UniProt ID: (Human) P41743, (Mouse) Q62074
Entrez Gene ID: (Human) 5584, (Mouse) 18759
protein kinase activity
protein serine/threonine kinase activity
protein kinase C activity
protein binding
ATP binding
phospholipid binding
protein domain specific binding
metal ion binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein phosphorylation
protein targeting to membrane
cytoskeleton organization
actin filament organization
positive regulation of neuron projection development
vesicle-mediated transport
cell migration
peptidyl-serine phosphorylation
cellular response to insulin stimulus
negative regulation of glial cell apoptotic process
establishment of apical/basal cell polarity
intracellular signal transduction
eye photoreceptor cell development
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
establishment or maintenance of epithelial cell apical/basal polarity
cell-cell junction organization
positive regulation of glucose import
secretion
Golgi vesicle budding
positive regulation of NF-kappaB transcription factor activity
positive regulation of glial cell proliferation
membrane organization
response to interleukin-1
bicellular tight junction assembly
positive regulation of establishment of protein localization to plasma membrane
positive regulation of endothelial cell apoptotic process
multicellular organism development
phosphorylation
cellular protein localization
response to peptide hormone
