Invitrogen

Phospho-ROCK2 (Ser1366) Polyclonal Antibody

Phospho-ROCK2 (Ser1366) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-ROCK2 (Ser1366) Antibody (PA5-34895) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Phospho-ROCK2-(Ser1366) was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: Phospho-ROCK2 (Ser1366) Polyclonal Antibody (Product # PA5-34895) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Phospho-ROCK2 (Ser1366) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-ROCK2 (Ser1366) Antibody in Western Blot (WB)

Phospho-ROCK2 (Ser1366) Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-34895

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phospho-peptide corresponding to amino acids residues surrounding the phospho Ser1366 of ROCK2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2552247

产品详细信息

Recommended positive controls: HeLa, 293T, 293T transfected with wild type (WT) plasmids.

Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ROCK2, the predominant ROCK isoform in skeletal muscle, is an important regulator downstream of Rho GTPases. ROCK2 regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Probing Anti-inflammatory Properties Independent of NF-κB Through Conformational Constraint of Peptide-Based Interleukin-1 Receptor Biased Ligands.

Frontiers in chemistry

Geranurimi A,Cheng CWH,Quiniou C,Zhu T,Hou X,Rivera JC,St-Cyr DJ,Beauregard K,Bernard-Gauthier V,Chemtob S,Lubell WD

PA5-34895 was used in Western Blotting to develop immunomodulatory therapies by use of small peptide mimic prototypes with easier administration and maintenance of beneficial effects of NF-κB signaling.

Thu Oct 01 00:00:00 EDT 2020

Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth.

Journal of immunology (Baltimore, Md. : 1950)

Nadeau-Vallée M,Quiniou C,Palacios J,Hou X,Erfani A,Madaan A,Sanchez M,Leimert K,Boudreault A,Duhamel F,Rivera JC,Zhu T,Noueihed B,Robertson SA,Ni X,Olson DM,Lubell W,Girard S,Chemtob S

PA5-34895 was used in Western Blot to demonstrate the efficacy of a small noncompetitive (all-d peptide) IL-1R-biased ligand, termed rytvela (labeled 101.10) in delaying IL-1β-, TLR2-, and TLR4-induced PTB in mice.

Thu Oct 01 00:00:00 EDT 2015

The effect of antenatal depression and selective serotonin reuptake inhibitor treatment on nerve growth factor signaling in human placenta.

PloS one

Kaihola H,Olivier J,Poromaa IS,Åkerud H

Published figure using Phospho-ROCK2 (Ser1366) polyclonal antibody (Product # PA5-34895) in Immunohistochemistry

Wed Sep 16 00:00:00 EDT 2015

The effect of antenatal depression and selective serotonin reuptake inhibitor treatment on nerve growth factor signaling in human placenta.

PloS one

Kaihola H,Olivier J,Poromaa IS,Åkerud H

Published figure using Phospho-ROCK2 (Ser1366) polyclonal antibody (Product # PA5-34895) in Immunohistochemistry

Wed Sep 16 00:00:00 EDT 2015

生物信息学

蛋白别名: p150 ROK-alpha; p164 ROCK-2; Rho kinase 2; Rho-associated coiled-coil forming kinage 2; Rho-associated coiled-coil forming kinase 2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; RhoA - binding serine/threosine kinase alpha (ROK - alpha); RhoA-binding kinase 2; ROCK II; ROCK-II; rockii; ROKalpha

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基因别名: B230113H15Rik; KIAA0619; mKIAA0619; Rho-kinase; ROCK-II; ROCK2; Rock2m; ROK; ROKalpha

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UniProt ID: (Human) O75116, (Rat) Q62868, (Mouse) P70336

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Entrez Gene ID: (Human) 9475, (Rat) 25537, (Mouse) 19878

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