Invitrogen

Phospho-Rb (Thr821) Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

9个已发表图片

16 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

Phospho-Rb (Thr821) Antibody in Western Blot (WB)

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Phospho-Rb (Thr821) Antibody (44-582G) in WB

Western Blot using Rabbit anti RB (pT821) polyclonal antibody.

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产品信息

44-582G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫沉淀 (IP)

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Rb that contains threonine 821 (based on Swiss Protein database, accession number P06400). The sequence is conserved in mouse and rat.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533685

靶标信息

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Cyclin A2 regulates erythrocyte morphology and numbers.

Cell cycle (Georgetown, Tex.)

Jayapal SR,Ang HY,Wang CQ,Bisteau X,Caldez MJ,Xuan GX,Yu W,Tergaonkar V,Osato M,Lim B,Kaldis P

Published figure using Phospho-Rb (Thr821) polyclonal antibody (Product # 44-582G) in Western Blot

Wed Nov 16 00:00:00 EST 2016

Molecular Determinants for the Inactivation of the Retinoblastoma Tumor Suppressor by the Viral Cyclin-dependent Kinase UL97.

The Journal of biological chemistry

Iwahori S,Hakki M,Chou S,Kalejta RF

Published figure using Phospho-Rb (Thr821) polyclonal antibody (Product # 44-582G) in Western Blot

Fri Aug 07 00:00:00 EDT 2015

Poldip2 knockout results in perinatal lethality, reduced cellular growth and increased autophagy of mouse embryonic fibroblasts.

PloS one

Brown DI,Lassègue B,Lee M,Zafari R,Long JS,Saavedra HI,Griendling KK

44-582G was used in western blot to demonstrate a novel role for Poldip2 in regulating the cell cycle

Thu Jan 29 00:00:00 EST 2015

Prenylated Flavonoids from Morus alba L. Cause Inhibition of G1/S Transition in THP-1 Human Leukemia Cells and Prevent the Lipopolysaccharide-Induced Inflammatory Response.

Evidence-based complementary and alternative medicine : eCAM

Kollar P,Bárta T,Hošek J,Souček K,Závalová VM,Artinian S,Talhouk R,Smejkal K,Suchý P,Hampl A

Published figure using Phospho-Rb (Thr821) polyclonal antibody (Product # 44-582G) in Western Blot

Fri Jun 14 00:00:00 EDT 2013

Geranylated flavanone tomentodiplacone B inhibits proliferation of human monocytic leukaemia (THP-1) cells.

British journal of pharmacology

Kollár P,Bárta T,Závalová V,Smejkal K,Hampl A

Fri Apr 01 00:00:00 EDT 2011

The cyclin-dependent kinase inhibitor PHA-848125 suppresses the in vitro growth of human melanomas sensitive or resistant to temozolomide, and shows synergistic effects in combination with this triazene compound.

Pharmacological research

Caporali S,Alvino E,Starace G,Ciomei M,Brasca MG,Levati L,Garbin A,Castiglia D,Covaciu C,Bonmassar E,D'Atri S

44-582G was used in western blot to present the effect of PHA-848125 on human melanoma cells in vitro

Sat May 01 00:00:00 EDT 2010

Cyclin-dependent kinase-like function is shared by the beta- and gamma- subset of the conserved herpesvirus protein kinases.

PLoS pathogens

Kuny CV,Chinchilla K,Culbertson MR,Kalejta RF

Thu Sep 09 00:00:00 EDT 2010

Dual targeting of CDK and tropomyosin receptor kinase families by the oral inhibitor PHA-848125, an agent with broad-spectrum antitumor efficacy.

Molecular cancer therapeutics

Albanese C,Alzani R,Amboldi N,Avanzi N,Ballinari D,Brasca MG,Festuccia C,Fiorentini F,Locatelli G,Pastori W,Patton V,Roletto F,Colotta F,Galvani A,Isacchi A,Moll J,Pesenti E,Mercurio C,Ciomei M

Sun Aug 01 00:00:00 EDT 2010

The kinase inhibitor O6-cyclohexylmethylguanine (NU2058) potentiates the cytotoxicity of cisplatin by mechanisms that are independent of its effect upon CDK2.

Biochemical pharmacology

Harrison LR,Ottley CJ,Pearson DG,Roche C,Wedge SR,Dolan ME,Newell DR,Tilby MJ

Fri May 15 00:00:00 EDT 2009

Acetylation by GCN5 regulates CDC6 phosphorylation in the S phase of the cell cycle.

Nature structural & molecular biology

Paolinelli R,Mendoza-Maldonado R,Cereseto A,Giacca M

Wed Apr 01 00:00:00 EDT 2009

Combined loss of Cdk2 and Cdk4 results in embryonic lethality and Rb hypophosphorylation.

Developmental cell

Berthet C,Klarmann KD,Hilton MB,Suh HC,Keller JR,Kiyokawa H,Kaldis P

44-582G was used in Western Blot to demonstrate that Cdk2 and Cdk4 cooperate to phosphorylate Rb in vivo and to couple the G1/S phase transition to mitosis via E2F-dependent regulation of gene expression.

Mon May 01 00:00:00 EDT 2006

Combined loss of Cdk2 and Cdk4 results in embryonic lethality and Rb hypophosphorylation.

Developmental cell

Berthet C,Klarmann KD,Hilton MB,Suh HC,Keller JR,Kiyokawa H,Kaldis P

Mon May 01 00:00:00 EDT 2006

Downregulation of Fer induces PP1 activation and cell-cycle arrest in malignant cells.

Oncogene

Pasder O,Shpungin S,Salem Y,Makovsky A,Vilchick S,Michaeli S,Malovani H,Nir U

44-582G was used in western blot to assess induction of PP1 activation and cell-cycle arrest in malignant cells by downregulation of Fer

Thu Jul 13 00:00:00 EDT 2006

Catalytic activities of G1 cyclin-dependent kinases and phosphorylation of retinoblastoma protein in mobilized peripheral blood CD34+ hematopoietic progenitor cells.

Stem cells (Dayton, Ohio)

Leibundgut K,Schmitz NM,Hirt A

Published figure using Phospho-Rb (Thr821) polyclonal antibody (Product # 44-582G) in Western Blot

Mon Aug 01 00:00:00 EDT 2005

ICI182,780 induces p21Waf1 gene transcription through releasing histone deacetylase 1 and estrogen receptor alpha from Sp1 sites to induce cell cycle arrest in MCF-7 breast cancer cell line.

The Journal of biological chemistry

Varshochi R,Halim F,Sunters A,Alao JP,Madureira PA,Hart SM,Ali S,Vigushin DM,Coombes RC,Lam EW

Fri Feb 04 00:00:00 EST 2005

ICI182,780 induces p21Waf1 gene transcription through releasing histone deacetylase 1 and estrogen receptor alpha from Sp1 sites to induce cell cycle arrest in MCF-7 breast cancer cell line.

The Journal of biological chemistry

Varshochi R,Halim F,Sunters A,Alao JP,Madureira PA,Hart SM,Ali S,Vigushin DM,Coombes RC,Lam EW

Fri Feb 04 00:00:00 EST 2005

Oxidative stress induces protein phosphatase 2A-dependent dephosphorylation of the pocket proteins pRb, p107, and p130.

The Journal of biological chemistry

Cicchillitti L,Fasanaro P,Biglioli P,Capogrossi MC,Martelli F

44-582G was used in western blot to investigate the roles of pRb, p107, and p130 in the cellular response to oxidative stress.

Fri May 23 00:00:00 EDT 2003

生物信息学

蛋白别名: Cleaved Rb; delta RB-p70; DRb-p70; exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; p105-Rb; P105RB; p110-RB1; Phospho-Retinoblastoma; pp110; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; Rb-p70; Retin; Retinoblastoma; retinoblastoma 1; retinoblastoma gene; retinoblastoma suspectibility protein; Retinoblastoma-associated protein

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基因别名: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB; RB1

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UniProt ID: (Human) P06400

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Entrez Gene ID: (Human) 5925

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功能