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Invitrogen
Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (G.22.6)
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
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Phospho-S6 (Ser235, Ser236) Antibody (MA5-15140) in ICC/IF
Immunofluorescence analysis of Phospho-S6 (Ser235,Ser236) was performed using 70% confluent log phase U-2 OS cells treated with 50 ng/mL IGF-1 for 6hrs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-S6 (Ser235,Ser236) Monoclonal Antibody (Product #... View More
产品信息
MA5-15140
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
G.22.6
抗原
Synthetic phosphopeptide corresponding to residues surrounding pSer235 and pSer236 of human ribosomal protein S6
偶联物
形式
Liquid
浓度
72 µg/mL
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
<0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_10977376
产品详细信息
It is not recommended to aliquot this antibody.
靶标信息
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 40S ribosomal protein S6; OTTHUMP00000021121; OTTHUMP00000021122; Phosphoprotein NP33; RP11-513M16.6; RS6; Small ribosomal subunit protein eS6
基因别名: OK/SW-cl.2; RPS6; S6; S6R
UniProt ID: (Human) P62753, (Mouse) P62754, (Rat) P62755
Entrez Gene ID: (Human) 6194, (Mouse) 20104, (Rat) 29304
G1/S transition of mitotic cell cycle
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
placenta development
T cell proliferation involved in immune response
rRNA processing
translation
translational initiation
SRP-dependent cotranslational protein targeting to membrane
activation-induced cell death of T cells
mitotic nuclear division
mitotic cell cycle checkpoint
gastrulation
viral transcription
oogenesis stage
TOR signaling
T cell differentiation in thymus
ribosomal small subunit biogenesis
glucose homeostasis
positive regulation of apoptotic process
negative regulation of apoptotic process
erythrocyte development
ribosomal small subunit assembly
