Invitrogen

Phospho-SMAD2/SMAD3 (Ser465, Ser467, Ser423, Ser425) Recombinant Rabbit Monoclonal Antibody (ST0493)

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实验方案
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产品信息

MA5-56386

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:2,000-1:5,000
-

免疫组化（石蜡） (IHC (P))

1:200-1:500
-

免疫细胞化学 (ICC/IF)

1:500
-
产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

ST0493

抗原

Synthetic phospho-peptide corresponding to residues surrounding Ser423 and 425 of human Smad3.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

产品详细信息

Positive Control: A549 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate, C2C12 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate, HeLa starved overnight then treated with 10ng/mL TGF-beta1 for 30 minutes cell lysate, NIH/3T3 starved overnight then treated with 10ng/mL TGF-beta1 for 30 minutes cell lysate, A549, human large intestine tissue, mouse skin tissue, mouse kidney tissue.

Tissue Specificity: Low tissue specificity.

Subcellular Location: Cytoplasm, Nucleus.

Sequence Similarities: 100% Mouse.

Predicted band size: 48 kDa.

靶标信息

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: hMAD-2; hMAD-3; hSMAD3; JV15-2; JV18-1; MAD homolog 2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; Mad-related protein 2; mad3; mMad2; mMad3; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 2; mothers against DPP homolog 3; pSMAD2; Sma- and Mad-related protein 2; SMA- and MAD-related protein 3; SMAD; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3; SMAD, mothers against DPP homolog 2; SMAD, mothers against DPP homolog 3

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基因别名: 7120426M23Rik; AU022421; hMAD-2; hSMAD2; HSPC193; HsT17436; JV15-2; JV18; JV18-1; LDS1C; LDS3; MADH2; MADH3; MADR2; mMad2; Smad-2; SMAD2; SMAD3

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UniProt ID: (Human) Q15796, (Human) P84022, (Mouse) Q62432, (Mouse) Q8BUN5

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Entrez Gene ID: (Human) 4087, (Human) 4088, (Mouse) 17126, (Mouse) 17127

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功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding transcription factor binding phosphatase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity ubiquitin protein ligase binding activating transcription factor binding type I transforming growth factor beta receptor binding enhancer binding protein homodimerization activity SMAD binding metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding R-SMAD binding transcription factor activity, RNA polymerase II core promoter sequence-specific core promoter proximal region sequence-specific DNA binding transcription factor activity, protein binding RNA polymerase II activating transcription factor binding collagen binding zinc ion binding protein kinase binding chromatin DNA binding identical protein binding ubiquitin binding bHLH transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding beta-catenin binding enzyme binding DNA-binding transcription factor gene-specific transcriptional regulator
参与通路
negative regulation of transcription from RNA polymerase II promoter ureteric bud development in utero embryonic development endoderm formation mesoderm formation transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly zygotic specification of dorsal/ventral axis gastrulation negative regulation of cell proliferation response to glucose post-embryonic development anterior/posterior pattern specification positive regulation of epithelial to mesenchymal transition regulation of transforming growth factor beta receptor signaling pathway signal transduction involved in regulation of gene expression insulin secretion lung development negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of BMP signaling pathway pancreas development primary miRNA processing activin receptor signaling pathway somatic stem cell population maintenance organ growth intracellular signal transduction nodal signaling pathway cell fate commitment negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of binding palate development pericardium development SMAD protein signal transduction response to cholesterol positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry response to hypoxia somitogenesis liver development heart looping osteoblast development immune system development transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transport activation of cysteine-type endopeptidase activity involved in apoptotic process immune response cell cycle arrest endoderm development embryonic pattern specification positive regulation of gene expression positive regulation of alkaline phosphatase activity regulation of striated muscle tissue development evasion or tolerance of host defenses by virus negative regulation of cell growth positive regulation of cell migration positive regulation of bone mineralization thyroid gland development positive regulation of chondrocyte differentiation positive regulation of interleukin-1 beta production regulation of transforming growth factor beta2 production positive regulation of transforming growth factor beta3 production negative regulation of osteoblast proliferation wound healing T cell activation positive regulation of transcription factor import into nucleus negative regulation of apoptotic process cell-cell junction organization negative regulation of fat cell differentiation negative regulation of osteoblast differentiation negative regulation of mitotic cell cycle developmental growth regulation of epithelial cell proliferation negative regulation of inflammatory response regulation of immune response positive regulation of positive chemotaxis positive regulation of stress fiber assembly positive regulation of focal adhesion assembly transdifferentiation negative regulation of wound healing lens fiber cell differentiation extrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway positive regulation of extracellular matrix assembly regulation of transcription, DNA-templated protein phosphorylation pattern specification process heart development negative regulation of gene expression skeletal system development osteoblast differentiation negative regulation of protein phosphorylation positive regulation of catenin import into nucleus negative regulation of protein catabolic process protein stabilization positive regulation of canonical Wnt signaling pathway
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