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Phospho-SREBP1 (Ser338) Antibody (PA5-105567) in ICC/IF
Immunocytochemistry analysis of Phospho-SREBP1 (Ser338) in A549 cells (H2O2 treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-SREBP1 (Ser338) polyclonal antibody (Product # PA5-105567) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG... View More
产品信息
PA5-105567
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human SREBF1(Accession P36956), corresponding to amino acid residues around phosphorylated Ser338.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2816995
产品详细信息
Antibody detects endogenous levels of SREBP1 only when phosphorylated at Ser338.
靶标信息
Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ADD1; Adipocyte determination- and differentiation-dependent factor 1; bHLHd1; Class D basic helix-loop-helix protein 1; nt-SREBP1; SREBP-1; Sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1
基因别名: ADD-1; ADD1; BHLHD1; D630008H06; SREBF1; SREBP-1; SREBP-1a; SREBP-1c; SREBP1; SREBP1a; SREBP1c
UniProt ID: (Human) P36956, (Rat) P56720, (Mouse) Q9WTN3
Entrez Gene ID: (Human) 6720, (Rat) 78968, (Mouse) 20787
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein kinase binding
protein complex binding
sterol response element binding
protein dimerization activity
sequence-specific DNA binding
transcription regulatory region DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of heart rate by chemical signal
regulation of transcription from RNA polymerase II promoter
lipid metabolic process
aging
circadian rhythm
cholesterol metabolic process
insulin receptor signaling pathway
lipid biosynthetic process
cellular response to starvation
response to glucose
positive regulation of triglyceride biosynthetic process
regulation of fatty acid metabolic process
lung development
positive regulation of histone deacetylation
regulation of protein stability
response to food
response to retinoic acid
response to progesterone
response to glucagon
response to drug
mRNA transcription from RNA polymerase II promoter
fat cell differentiation
positive regulation of cholesterol biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin secretion
response to cAMP
cellular response to fatty acid
regulation of mitophagy
regulation of protein targeting to mitochondrion
transcription, DNA-templated
regulation of transcription, DNA-templated
response to organic cyclic compound
response to insulin
cellular response to insulin stimulus
response to lipid
response to peptide hormone
positive regulation of transcription, DNA-templated
regulation of insulin secretion
response to fatty acid
transcription from RNA polymerase II promoter
steroid metabolic process
positive regulation of fatty acid biosynthetic process
