Invitrogen

Phospho-STAT1 (Tyr701) Monoclonal Antibody (KIKSI0803), PE, eBioscience™

Phospho-STAT1 (Tyr701) Antibody in Flow Cytometry (Flow)

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Phospho-STAT1 (Tyr701) Antibody (12-9008-42) in Flow

TOP: Intracellular staining of unstimulated (left) or 15-minute IFN gamma-stimulated (right) normal human peripheral blood cells with Anti-Human CD4 eFluor® 450 (Product # 48-0047-42) and Anti-Human phospho-STAT1 (Y701) PE using the Fixation/Methanol Protocol. Monocytes are defined in blue, CD4+ T cells are defined in green. BOTTOM: Intracellular staining of unstimulated (orange histogram) or 15-minute IFN ... View More

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产品信息

12-9008-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

KIKSI0803

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2572669

产品详细信息

Description: This KIKSI0803 monoclonal antibody recognizes signal transducer and activator of transcription 1 (STAT1) when phosphorylated on tyrosine 701. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. Phosphorylation of STAT1 on tyrosine 701 by JAK1 and JAK2 is essential for STAT1 dimer formation, nuclear translocation, and DNA binding activity. In response to IFN gamma stimulation, STAT1 homodimerizes or forms heterodimers with STAT3 that can bind to GAS (IFN gamma-activated sequence) promoter elements. In response to either IFN alpha or IFN beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind ISRE (IFN-stimulated response element) promoter elements.

Specificity of this KIKSI0803 clone was determined by ELISA, flow cytometry, and western blotting.

Applications Reported: This KIKSI0803 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This KIKSI0803 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Hematopoietic stem cell heterogeneity is linked to the initiation and therapeutic response of myeloproliferative neoplasms.

Cell stem cell

Tong J,Sun T,Ma S,Zhao Y,Ju M,Gao Y,Zhu P,Tan P,Fu R,Zhang A,Wang D,Wang D,Xiao Z,Zhou J,Yang R,Loughran SJ,Li J,Green AR,Bresnick EH,Wang D,Cheng T,Zhang L,Shi L

Published figure using Phospho-STAT1 (Tyr701) monoclonal antibody (Product # 12-9008-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Mapping of Signaling Pathways Linked to sIgAD Reveals Impaired IL-21 Driven STAT3 B-Cell Activation.

Frontiers in immunology

Lemarquis AL,Theodors F,Einarsdottir HK,Ludviksson BR

12-9008-42 was used in Flow cytometry/Cell sorting to delineate complex intracellular signaling pathways and their potential role in the immunopathology linked to sIgAD.

Tue Jul 14 00:00:00 EDT 2020

Autoimmunity, hypogammaglobulinemia, lymphoproliferation, and mycobacterial disease in patients with activating mutations in STAT3.

Blood

Haapaniemi EM,Kaustio M,Rajala HL,van Adrichem AJ,Kainulainen L,Glumoff V,Doffinger R,Kuusanmäki H,Heiskanen-Kosma T,Trotta L,Chiang S,Kulmala P,Eldfors S,Katainen R,Siitonen S,Karjalainen-Lindsberg ML,Kovanen PE,Otonkoski T,Porkka K,Heiskanen K,Hänninen A,Bryceson YT,Uusitalo-Seppälä R,Saarela J,Seppänen M,Mustjoki S,Kere J

12-9008 was used in Flow cytometry/Cell sorting to investigate phenotypes caused by activating STAT3 mutations, linking understanding of the STAT family of TFs and autoimmunity.

Thu Jan 22 00:00:00 EST 2015

Autoimmunity, hypogammaglobulinemia, lymphoproliferation, and mycobacterial disease in patients with activating mutations in STAT3.

Blood

12-9008 was used in Flow cytometry/Cell sorting to investigate phenotypes caused by activating STAT3 mutations, linking understanding of the STAT family of TFs and autoimmunity.

Thu Jan 22 00:00:00 EST 2015

生物信息学

蛋白别名: DKFZp686B04100; OTTHUMP00000165046; OTTHUMP00000205845; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1-alpha/beta; signal transducer and activator of transcription-1; STAT; STAT 1; STAT-1; stat1 alpha; Transcription factor ISGF-3 components p91/p84

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基因别名: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91

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UniProt ID: (Human) P42224

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Entrez Gene ID: (Human) 6772

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功能