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Invitrogen
Phospho-Smad5 (Ser463,Ser465) Recombinant Rabbit Monoclonal Antibody (ARC1568)
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Phospho-Smad5 (Ser463,Ser465) Antibody (MA5-35867) in WB
Western blot analysis of Phospho-Smad5 (Ser463,Ser465) in extracts of HeLa cells. Samples were incubated with Phospho-Smad5 (Ser463,Ser465) Monoclonal antibody (Product # MA5-35867) using a dilution of 1:1,000 or Smad5 antibody (Product # PA5-120676). Followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Hela cells were treated by ATP (5 mM) at 30°C for 1 hour. Lysates/pro... View More
产品信息
MA5-35867
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
ARC1568
抗原
A synthetic phosphorylated peptide around S463 & S465 of human Smad5 (Q99717)
偶联物
形式
Liquid
浓度
0.34 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2849765
产品详细信息
Immunogen sequence: SSVS
靶标信息
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp781C1895; DKFZp781O1323; hSmad5; JV5-1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; OTTHUMP00000223331; SMA- and MAD-related protein 5; SMAD; SMAD 5; SMAD family member 5; SMAD, mothers against DPP homolog 5
基因别名: DWFC; JV5-1; MADH5; SMAD5
UniProt ID: (Human) Q99717
Entrez Gene ID: (Human) 4090
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding
receptor signaling protein activity
protein binding
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
ubiquitin protein ligase binding
metal ion binding
DNA-binding transcription factor
gene-specific transcriptional regulator
ureteric bud development
Mullerian duct regression
osteoblast fate commitment
transcription, DNA-templated
protein phosphorylation
signal transduction
transforming growth factor beta receptor signaling pathway
germ cell development
embryonic pattern specification
erythrocyte differentiation
BMP signaling pathway
positive regulation of osteoblast differentiation
positive regulation of transcription, DNA-templated
cartilage development
cardiac muscle contraction
bone development
SMAD protein signal transduction
cellular response to organic cyclic compound
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
