Invitrogen

Phospho-TSC1 (Ser312) Polyclonal Antibody

Phospho-TSC1 (Ser312) Antibody in Dot Blot (DB)

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Phospho-TSC1 (Ser312) Antibody (PA5-72380) in DB

Dot blot analysis of Phospho-TSC1 (Ser312). Samples were incubated with Phospho-TSC1 (Ser312) polyclonal antibody (Product # PA5-72380) using a dilution of 0.6 µg/mL. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed on nitrocellulose membrane.

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产品信息

PA5-72380

应用

建议稀释比

已发表文章

点杂交 (DB)

1:1,000

产品规格

种属反应

Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S312 of rat TSC1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.32 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2718234

靶标信息

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

仅用于科研。不用于诊断过程。未经明确授权不得转售。