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Phospho-TSC2 (Ser1387) Antibody (PA5-105177) in ICC/IF
Immunocytochemistry analysis of Phospho-TSC2 (Ser1387) in A549 cells (H2O2 treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-TSC2 (Ser1387) polyclonal antibody (Product # PA5-105177) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (... View More
产品信息
PA5-105177
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human TSC2(Accession P49815), corresponding to amino acid residues around phosphorylated Ser1387.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2816650
产品详细信息
Antibody detects endogenous levels of Tuberin/TSC2 only when phosphorylated at Ser1387.
靶标信息
TSC2 (Tuberin, Tuberous sclerosis complex, TSC Complex Subunit 2), is implicated as a tumor suppressor and may function in vesicular transport, play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. TSC2 associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Phosphorylation of tuberin on Ser 939 and Thr 1462 regulates its interaction with hamartin, and is stimulated by various growth factors through the phosphoinositide 3-kinase/Akt pathway. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking. TSC2 specifically stimulates the intrinsic GTPase activity of the Ras related protein RAP1A and RAB5, suggesting a possible mechanism for its role in regulating cellular growth. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors and are involved in diseases such as lymphangioleiomyomatosis and TSC2 angiomyolipoma. Alternative splicing results in transcript variants of three different isoforms of TSC2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: OTTHUMP00000198394; protein phosphatase 1, regulatory subunit 160; renal carcinoma; Tsc2; Tuberin; tuberous sclerosis 2 homolog protein; Tuberous sclerosis 2 protein; Tuberous sclerosis 2 protein homolog
基因别名: LAM; Nafld; PPP1R160; Rc; Tcs2; TSC2; TSC4
UniProt ID: (Human) P49815, (Rat) P49816
Entrez Gene ID: (Human) 7249, (Rat) 24855, (Mouse) 22084
neural tube closure
negative regulation of protein kinase activity
protein import into nucleus
endocytosis
cell cycle arrest
heart development
protein localization
negative regulation of cell proliferation
negative regulation of phosphatidylinositol 3-kinase signaling
viral process
vesicle-mediated transport
regulation of endocytosis
negative regulation of Wnt signaling pathway
negative regulation of TOR signaling
protein kinase B signaling
positive regulation of GTPase activity
regulation of insulin receptor signaling pathway
negative regulation of insulin receptor signaling pathway
insulin-like growth factor receptor signaling pathway
positive chemotaxis
regulation of small GTPase mediated signal transduction
regulation of cell cycle
negative regulation of protein kinase B signaling
response to hypoxia
acute-phase response
signal transduction
phosphatidylinositol 3-kinase signaling
cell projection organization
cellular response to insulin stimulus
negative regulation of MAP kinase activity
protein transport into plasma membrane raft
establishment of protein localization
negative regulation of cell size
positive regulation of transcription from RNA polymerase II promoter
glucose import
negative regulation of epithelial cell proliferation
protein homooligomerization
protein heterooligomerization
