Invitrogen

Phospho-c-Jun (Ser63) Polyclonal Antibody

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

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1 篇参考文献
查看其他55个c-Jun抗体
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产品信息

PA5-17890

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000
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免疫细胞化学 (ICC/IF)

1:100
-

免疫沉淀 (IP)

1:50
-
产品规格

种属反应

Human, Mouse, Non-human primate, Pig, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phosphopeptide corresponding to residues around pSer63 of human c-Jun
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

153 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10980910

产品详细信息

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

It is not recommended to aliquot this antibody.

This antibody does not recognize the phosphorylated forms of JunD or JunB.

靶标信息

c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Activator protein 1; AH119; AP1; Avian sarcoma virus 17 (v-jun) oncogene homolog; cjun; enhancer-binding protein AP1; immediate early; Jun A; Jun activation domain binding protein; Jun oncogene; jun proto-oncogene; JUND; p39; Proto-oncogene c-Jun; transcription factor; transcription factor AP-1; Transcription factor AP-1 subunit Jun; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog

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基因别名: AP-1; AP1; C-JUN; JUN; Junc; Rjg-9

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UniProt ID: (Pig) P56432, (Human) P05412, (Rat) P17325, (Mouse) P05627

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Entrez Gene ID: (Pig) 396913, (Human) 3725, (Rat) 24516, (Mouse) 16476

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功能
transcription regulatory region sequence-specific DNA binding DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity GTPase activator activity protein binding transcription factor binding enzyme binding cAMP response element binding identical protein binding protein homodimerization activity transcription regulatory region DNA binding poly(A) RNA binding protein heterodimerization activity R-SMAD binding HMG box domain binding double-stranded DNA binding sequence-specific DNA binding basic leucine zipper transcription factor
参与通路
immune response Ras protein signal transduction positive regulation of transcription, DNA-templated positive regulation of sequence-specific DNA binding transcription factor activity cellular response to lipopolysaccharide angiogenesis microglial cell activation release of cytochrome c from mitochondria liver development positive regulation of endothelial cell proliferation outflow tract morphogenesis transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway SMAD protein import into nucleus aging learning circadian rhythm negative regulation of cell proliferation response to radiation response to mechanical stimulus positive regulation of epithelial cell migration regulation of cell death monocyte differentiation axon regeneration negative regulation of protein autophosphorylation response to lipopolysaccharide cellular response to hormone stimulus response to cytokine leading edge cell differentiation response to muscle stretch Fc-epsilon receptor signaling pathway regulation of cell proliferation response to drug response to hydrogen peroxide negative regulation of DNA binding negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process positive regulation of GTPase activity negative regulation by host of viral transcription positive regulation by host of viral transcription positive regulation of cell differentiation positive regulation of monocyte differentiation positive regulation of DNA replication negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation positive regulation of smooth muscle cell proliferation regulation of sequence-specific DNA binding transcription factor activity cellular response to potassium ion starvation response to cAMP regulation of cell cycle membrane depolarization SMAD protein signal transduction eyelid development in camera-type eye positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress positive regulation of DNA-templated transcription, initiation transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of cell proliferation cellular process response to organic substance response to organic cyclic compound positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of transcription from RNA polymerase II promoter
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