Invitrogen

Phospho-c-Jun (Thr91) Recombinant Rabbit Monoclonal Antibody (ARC1548)

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抗体检测数据 (3)

Phospho-c-Jun (Thr91) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-c-Jun (Thr91) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-c-Jun (Thr91) Antibody (MA5-35856) in IHC (P)

Immunohistochemistry analysis of Phospho-c-Jun (Thr91) in paraffin-embedded human breast. Samples were incubated with Phospho-c-Jun (Thr91) Monoclonal antibody (Product # MA5-35856) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Phospho-c-Jun (Thr91) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-c-Jun (Thr91) Antibody in Western Blot (WB)

Phospho-c-Jun (Thr91) Antibody in Western Blot (WB)

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Phospho-c-Jun (Thr91) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-c-Jun (Thr91) in paraffin-embedded human breast. Samples were incubated with Phospho-c-Jun (Thr91) Monoclonal antibody (Product # MA5-35856) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Phospho-c-Jun (Thr91) Antibody in Western Blot (WB) — Western blot analysis of Phospho-c-Jun (Thr91) in extracts of NIH/3T3 cells. Samples were incubated with Phospho-c-Jun (Thr91) Monoclonal antibody (Product # MA5-35856) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 µg/mL) at 37°C for 30 minutes. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Phospho-c-Jun (Thr91) Antibody in Western Blot (WB) — Western blot analysis of Phospho-c-Jun (Thr91) in extracts of 293T cells. Samples were incubated with Phospho-c-Jun (Thr91) Monoclonal antibody (Product # MA5-35856) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. 293T cells were treated by UV at room temperature for 15-30 minutes. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3 min.

产品信息

MA5-35856

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

ARC1548

抗原

A synthetic phosphorylated peptide around T91 of human c-Jun (P05412)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.9 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2849754

产品详细信息

Immunogen sequence: TTTPT

靶标信息

c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Activator protein 1; AH119; AP1; Avian sarcoma virus 17 (v-jun) oncogene homolog; cjun; enhancer-binding protein AP1; immediate early; Jun A; Jun activation domain binding protein; Jun oncogene; jun proto-oncogene; JUND; p39; Proto-oncogene c-Jun; transcription factor AP-1; Transcription factor AP-1 subunit Jun; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog

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基因别名: AP-1; AP1; c-Jun; JUN; Junc; Rjg-9

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UniProt ID: (Human) P05412, (Rat) P17325, (Mouse) P05627

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Entrez Gene ID: (Human) 3725, (Rat) 24516, (Mouse) 16476

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RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity GTPase activator activity protein binding transcription factor binding enzyme binding cAMP response element binding identical protein binding protein homodimerization activity transcription regulatory region DNA binding poly(A) RNA binding protein heterodimerization activity R-SMAD binding HMG box domain binding double-stranded DNA binding sequence-specific DNA binding basic leucine zipper transcription factor

参与通路

angiogenesis microglial cell activation release of cytochrome c from mitochondria liver development positive regulation of endothelial cell proliferation outflow tract morphogenesis transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway SMAD protein import into nucleus Ras protein signal transduction aging learning circadian rhythm negative regulation of cell proliferation response to radiation response to mechanical stimulus positive regulation of epithelial cell migration regulation of cell death monocyte differentiation axon regeneration negative regulation of protein autophosphorylation response to lipopolysaccharide cellular response to hormone stimulus response to cytokine leading edge cell differentiation response to muscle stretch Fc-epsilon receptor signaling pathway regulation of cell proliferation response to drug response to hydrogen peroxide negative regulation of DNA binding negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process positive regulation of GTPase activity negative regulation by host of viral transcription positive regulation by host of viral transcription positive regulation of cell differentiation positive regulation of monocyte differentiation positive regulation of DNA replication negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation positive regulation of smooth muscle cell proliferation regulation of sequence-specific DNA binding transcription factor activity cellular response to potassium ion starvation response to cAMP regulation of cell cycle membrane depolarization SMAD protein signal transduction eyelid development in camera-type eye positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress positive regulation of DNA-templated transcription, initiation transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of cell proliferation cellular process response to organic substance response to organic cyclic compound positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of transcription from RNA polymerase II promoter