Invitrogen

Phospho-eNOS (Ser1179) Polyclonal Antibody

Phospho-eNOS (Ser1179) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-eNOS (Ser1179) Antibody (36-9100) in IHC (P)

Immunohistochemistry analysis of Phospho-eNOS (pSer1179) showing staining in the membrane of paraffin-embedded human placenta tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at r... View More

Phospho-eNOS (Ser1179) Antibody in Western Blot (WB)

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产品信息

36-9100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

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酶联免疫吸附实验 (ELISA)

Assay-dependent

其他PubMed (Misc)

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产品规格

种属反应

Bovine

已发表种属

Bovine, Human, Mouse, Non-human primate, Pig, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the C-terminal region of the bovine eNOS (endothelial nitric oxide synthase, NOS III) protein, phosphorylated at Ser1179.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.01% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533285

靶标信息

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Low-Dose Tacrolimus Promotes the Migration and Invasion and Nitric Oxide Production in the Human-Derived First Trimester Extravillous Trophoblast Cells In Vitro.

International journal of molecular sciences

Albaghdadi AJH,Coyle K,Kan FWK

Published figure using Phospho-eNOS (Ser1179) polyclonal antibody (Product # 36-9100) in Western Blot

Fri Jul 29 00:00:00 EDT 2022

Tongxinluo reduces myocardial no-reflow and ischemia-reperfusion injury by stimulating the phosphorylation of eNOS via the PKA pathway.

American journal of physiology. Heart and circulatory physiology

Li XD,Yang YJ,Geng YJ,Jin C,Hu FH,Zhao JL,Zhang HT,Cheng YT,Qian HY,Wang LL,Zhang BJ,Wu YL

36-9100 was used in western blot to test if tongxinluo treatment before myocardial ischemia reduces ischemia-reperfusion injury

Fri Oct 01 00:00:00 EDT 2010

Quantitative proteomics of caveolin-1-regulated proteins: characterization of polymerase i and transcript release factor/CAVIN-1 IN endothelial cells.

Molecular & cellular proteomics : MCP

Dávalos A,Fernández-Hernando C,Sowa G,Derakhshan B,Lin MI,Lee JY,Zhao H,Luo R,Colangelo C,Sessa WC

36-9100 was used in Western Blotting to study the role of Caveolin-1 as a regulator of caveola function in endothelial cells.

Fri Oct 01 00:00:00 EDT 2010

Nitric oxide modulates murine yolk sac vasculogenesis and rescues glucose induced vasculopathy.

Development (Cambridge, England)

Nath AK,Enciso J,Kuniyasu M,Hao XY,Madri JA,Pinter E

Sat May 01 00:00:00 EDT 2004

Insulin resistance does not diminish eNOS expression, phosphorylation, or binding to HSP-90.

American journal of physiology. Heart and circulatory physiology

Fulton D,Harris MB,Kemp BE,Venema RC,Marrero MB,Stepp DW

Wed Dec 01 00:00:00 EST 2004

Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme.

The Journal of biological chemistry

Fulton D,Fontana J,Sowa G,Gratton JP,Lin M,Li KX,Michell B,Kemp BE,Rodman D,Sessa WC

Fri Feb 08 00:00:00 EST 2002

Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme.

The Journal of biological chemistry

Fulton D,Fontana J,Sowa G,Gratton JP,Lin M,Li KX,Michell B,Kemp BE,Rodman D,Sessa WC

Fri Feb 08 00:00:00 EST 2002

Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme.

The Journal of biological chemistry

Fulton D,Fontana J,Sowa G,Gratton JP,Lin M,Li KX,Michell B,Kemp BE,Rodman D,Sessa WC

Fri Feb 08 00:00:00 EST 2002

Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme.

The Journal of biological chemistry

Fulton D,Fontana J,Sowa G,Gratton JP,Lin M,Li KX,Michell B,Kemp BE,Rodman D,Sessa WC

Fri Feb 08 00:00:00 EST 2002

生物信息学

蛋白别名: cNOS; constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; endothelial NOS; eNOS; nitric oxide synthase 3 (endothelial cell); NOS type III; NOSIII; OTTHUMP00000213183

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Entrez Gene ID: (Bovine) 287024

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功能

nitric-oxide synthase activity calmodulin binding FMN binding heme binding metal ion binding flavin adenine dinucleotide binding NADP binding oxidoreductase

参与通路

arginine catabolic process nitric oxide biosynthetic process mitochondrion organization blood coagulation positive regulation of guanylate cyclase activity oxidation-reduction process cellular response to laminar fluid shear stress negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of leukocyte cell-cell adhesion