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Pleckstrin Antibody (MA5-55527) in WB
Western Blot analysis of various lysates using Pleckstrin Recombinant Monoclonal Antibody (Product # MA5-55527) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
产品信息
MA5-55527
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
4W2K3
抗原
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Pleckstrin.
偶联物
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
产品详细信息
Immunogen Sequence: MEPKRIREGY LVKKGSVFNT WKPMWVVLLE DGIEFYKKKS DNSPKGMIPL KGSTLTSPCQ DFGKRMFVFK ITTTKQQDHF FQAAFLEERD AWVRDIKKAI
靶标信息
Major protein kinase C substrate of platelets.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ27168; p47; Platelet 47 kDa protein; Pleckstrin
基因别名: 2010300B13Rik; P47; PLEK
UniProt ID: (Human) P08567, (Mouse) Q9JHK5
Entrez Gene ID: (Human) 5341, (Mouse) 56193
hematopoietic progenitor cell differentiation
platelet degranulation
vesicle docking involved in exocytosis
integrin-mediated signaling pathway
positive regulation of platelet activation
negative regulation of inositol phosphate biosynthetic process
positive regulation of inositol-polyphosphate 5-phosphatase activity
cell projection organization
positive regulation of actin filament depolymerization
phospholipase C-inhibiting G-protein coupled receptor signaling pathway
cortical actin cytoskeleton organization
ruffle organization
actin cytoskeleton reorganization
positive regulation of actin filament bundle assembly
positive regulation of integrin activation
negative regulation of G-protein coupled receptor protein signaling pathway
phosphatidylinositol metabolic process
negative regulation of calcium-mediated signaling
regulation of cell diameter
thrombin receptor signaling pathway
platelet aggregation
protein kinase C signaling
protein secretion by platelet
intracellular signal transduction
