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Application
FIGURE: 1 / 5
RAB10 Antibody (PA5-102855) in ICC/IF
Immunofluorescence analysis of RAB10 was performed using 70% confluent log phase SK-O-V3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAB10 Polyclonal Antibody (Product # PA5-102855) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790), (1:... View More
Product Details
PA5-102855
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RAB10(Accession P61026), corresponding to amino acid residues I157-C200.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2852241
Product Specific Information
Antibody detects endogenous levels of total Rab10.
Target Information
RAB10 belongs to the RAS superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GTP-binding protein RAB10; ras-related GTP-binding protein; Ras-related protein Rab-10; ras-related protein rab10
Gene Aliases: Ac1075; RAB10
UniProt ID: (Human) P61026
Entrez Gene ID: (Human) 10890, (Rat) 50993
intracellular protein transport
Golgi to plasma membrane transport
vesicle docking involved in exocytosis
small GTPase mediated signal transduction
axonogenesis
metabolic process
protein secretion
protein transport
vesicle-mediated transport
endosomal transport
regulation of exocytosis
antigen processing and presentation
polarized epithelial cell differentiation
Rab protein signal transduction
cellular response to insulin stimulus
Golgi to plasma membrane protein transport
establishment of neuroblast polarity
basolateral protein localization
cellular response to antibiotic
endoplasmic reticulum tubular network organization
protein localization to plasma membrane
establishment of protein localization to membrane
establishment of protein localization to endoplasmic reticulum membrane
membrane organization
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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