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RAB27A Antibody (PA5-47907) in ICC/IF
Immunocytochemical analysis of RAB27A was detected in immersion fixed U937 human histiocytic lymphoma cell line using Sheep Anti-human/mouse/Rat RAB27A Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47907) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Sheep IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
产品信息
PA5-47907
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Sheep
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived recombinant human RAB27A Ser135-Ala218
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2608619
产品详细信息
This antibody detects human, mouse, and rat RAB27A in Western blots and recombinant human RAB27A in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human RAB27B is observed.
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
靶标信息
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ashen; GTP-binding protein Ram; low Mr GTP-binding protein; mutant Ras-related protein Rab-27A; Rab-27; Ram p25; Ras-related protein Rab-27A
基因别名: 2210402C08Rik; 2410003M20Rik; 4933437C11Rik; ash; GS2; HsT18676; RAB27; RAB27A; RAM
UniProt ID: (Human) P51159, (Mouse) Q9ERI2, (Rat) P23640
Entrez Gene ID: (Human) 5873, (Mouse) 11891, (Rat) 50645
protein targeting
exocytosis
small GTPase mediated signal transduction
blood coagulation
positive regulation of gene expression
antigen processing and presentation
melanocyte differentiation
melanosome localization
melanosome transport
multivesicular body organization
cytotoxic T cell degranulation
natural killer cell degranulation
positive regulation of exocytosis
synaptic vesicle transport
positive regulation of phagocytosis
multivesicular body sorting pathway
complement-dependent cytotoxicity
positive regulation of regulated secretory pathway
positive regulation of reactive oxygen species biosynthetic process
positive regulation of constitutive secretory pathway
exosomal secretion
vesicle docking involved in exocytosis
protein secretion
protein transport
vesicle-mediated transport
Rab protein signal transduction
pigmentation
pigment granule localization
pigment granule transport
intracellular protein transport
metabolic process
