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Application
FIGURE: 1 / 5
RAB34 Antibody (PA5-99697) in ICC/IF
Immunocytochemistry analysis of RAB34 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RAB34 polyclonal antibody (Product # PA5-99697) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-99697
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RAB34(Accession Q9BZG1), corresponding to amino acid residues K144-Y194.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2818630
Product Specific Information
Antibody detects endogenous levels of total Rab 34.
Target Information
Protein transport. Involved in the redistribution of lysosomes to the peri-Golgi region. Plays a role in the maturation of phagosomes that engulf pathogens, such as S. aureus and M. tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Nine amino-acid residue-repeats; RAB34, member of RAS oncogene family; Ras-related protein Rab-34; Ras-related protein Rab-34, isoform NARR; Ras-related protein Rab-39; Ras-related protein RAB34; Ras-related protein Rah
Gene Aliases: NARR; RAB34; RAB39; RAH
UniProt ID: (Human) P0DI83, (Rat) Q5U1Y1
Entrez Gene ID: (Human) 83871, (Rat) 360571
small GTPase mediated signal transduction
antigen processing and presentation
lysosome localization
Golgi to plasma membrane protein transport
macropinocytosis
protein localization to plasma membrane
phagosome maturation
phagosome-lysosome fusion
intracellular protein transport
endocytosis
metabolic process
protein transport
Rab protein signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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