Invitrogen

RAB3A Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (6)
高级验证 (1)

RAB3A Antibody in Immunocytochemistry (ICC/IF)

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RAB3A Antibody (PA1-770) in ICC/IF

Immunofluorescence analysis of RAB3A was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RAB3A Rabbit Polyclonal Antibody (Product # PA1-770) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at... View More

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RAB3A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Relative expression of RAB3A was observed in Mouse Brain and Rat Brain in comparison to Mouse Kidney, Mouse Liver, Rat Kidney and Rat Liver using Anti-RAB3A Polyclonal Antibody (Product # PA1-770) in Western Blot. {RE}

产品信息

PA1-770

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

1:100-1:500

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Dog, Hamster, Human, Mouse, Rat

已发表种属

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues M(1) A S A T D S R Y G Q K E S S D Q N(18) C of human RAB3A.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2177381

产品详细信息

PA1-770 detects rab 3A from canine, hamster, human, mouse and rat tissues and cells.

PA1-770 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~21 kDa protein representing rab 3A from Hepa1 cell lysate.

PA1-770 immunogen is a synthetic peptide corresponding to residues M(1) A S A T D S R Y G Q K E S S D Q N(18) C of human RAB3A. This sequence is completely conserved between human, mouse, and rat rab 3A. PA1-770 immunizing peptide (Cat. # PEP-098) is available for use in neutralization and control experiments.

Recombinant human rab 3A (Cat # RP-770) protein can be purchased for control experiments in Western blot applications.

靶标信息

Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has 4 known isoforms: 3A, 3B, 3C, and 3D. These proteins are expressed in highest concentrations in brain and endocrine tissues and involved in regulated exocytosis of hormones and neurotransmitters. Specifically, Rab 3s are thought to play a key role in presynaptic vesicle trafficking and priming.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Secreted key regulators (Fgf1, Bmp4, Gdf3) are expressed by PAC1-immunopositive retinal ganglion cells in the postnatal rat retina.

European journal of histochemistry : EJH

Dénes V,Kovacs K,Lukáts Á,Mester A,Berta G,Szabó A,Gabriel R

PA1-770 was used in Immunohistochemistry to show that these proteins could regulate postnatal RGC development acting through intracrine pathways.

Wed Apr 27 00:00:00 EDT 2022

Systematic proteomic analysis of LRRK2-mediated Rab GTPase phosphorylation establishes a connection to ciliogenesis.

eLife

Steger M,Diez F,Dhekne HS,Lis P,Nirujogi RS,Karayel O,Tonelli F,Martinez TN,Lorentzen E,Pfeffer SR,Alessi DR,Mann M

PA1-770 was used in Western Blotting to assess the LRRK2-mediated phosphorylation of the Rab GTPase family to establish a connection to primary ciliogenesis.

Fri Nov 10 00:00:00 EST 2017

生物信息学

蛋白别名: C18A3.6; RAB3A, member RAS oncogene family; RAS-associated protein RAB3A; Ras-related protein Rab-3A; ras-related protein Rab-3A-like protein; Ras-related small GTP binding protein 3A

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基因别名: RAB3; RAB3A

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UniProt ID: (Human) P20336, (Rat) P63012, (Mouse) P63011