Invitrogen

RAB9 Recombinant Rabbit Monoclonal Antibody (SR45-05)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他14个RAB9抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (8)
高级验证 (1)

RAB9 Antibody in Immunocytochemistry (ICC/IF)

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RAB9 Antibody (MA5-31997) in ICC/IF

Immunofluorescence analysis of Rab9a was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAB9 Recombinant Rabbit Monoclonal Antibody (SR45-05) (Product # MA5-31997) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, ... View More

RAB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RAB9 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with Rab9a siRNA and decrease in signal intensity was observed in Western Blot application using Anti-RAB9 Recombinant Rabbit Monoclonal Antibody (SR45-05) (Product # MA5-31997). {KD}

产品信息

MA5-31997

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫组化（石蜡） (IHC (P))

1:50

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SR45-05

抗原

Synthetic peptide within Human Rab9 aa 31-80

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2809291

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。