Invitrogen

RAD50 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他20个RAD50抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (4)
高级验证 (2)

RAD50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RAD50 Antibody (PA5-31555) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Rad50 (Product # PA5-31555) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

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RAD50 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. Hela cells were transfected with RAD50 shRNA and decrease in signal intensity was observed in western blot application using anti RAD50 Polyclonal Antibody (Product # PA5-31555). {KD}

产品信息

PA5-31555

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 700 and 973 of Human Rad50

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2549028

产品详细信息

Recommended positive controls: HeLa, mouse testis, NT2D1, rat testis.

Predicted reactivity: Mouse (91%), Rat (91%), Rabbit (95%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。