Invitrogen

RAN Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他19个RAN抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (1)

RAN Antibody in Immunocytochemistry (ICC/IF)

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RAN Antibody (PA5-17652) in ICC/IF

Immunofluorescence analysis of RAN was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAN Polyclonal Antibody (Product # PA5-17652) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilutio... View More

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RAN Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RAN siRNA and decrease in signal intensity was observed in western blot application using Anti-RAN Polyclonal Antibody (Product # PA5-17652). {KD}

产品信息

PA5-17652

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:200

产品规格

种属反应

Non-human primate, Rat, Xenopus, Bovine, Fruit fly, Hamster, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Two synthetic peptides corresponding to amino acids surrounding Arg29 and Asn143 of human Ran

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10981803

产品详细信息

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other small GTPases of the Ras family.

靶标信息

Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Androgen receptor-associated protein 24; ARA25; CG1404-PA; CG1404-PB; CG1404-PC; CG1404-PD; GM14354p; GTP-binding nuclear protein Ran; GTPase Ran; guanosine triphosphatase Ran; LD32416p; LD40852p; member RAS oncogene family; OK/SW-cl.81; OTTHUMP00000166078; OTTHUMP00000166102; ran; ran GTP-binding protein; Ran GTPase; RAN, member RAS oncogene family; Ran-PA; Ran-PB; Ran-PC; Ran-PD; RanGTPase; Ras-like protein TC4; Ras-related nuclear protein

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基因别名: AAF30287; ARA24; CG1404; Dmel\CG1404; Dmel_CG1404; dran; Gsp1; I79_024397; l(1)G0075; OK/SW-cl.81; Q9VZ23; RAN; ran-1; Ran10A; RanGTP; RanTC4; Rasl2-8; TC4; XELAEV_18007741mg; xran