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RANBP2 Antibody (MA1-847) in ICC/IF
Immunofluorescent analysis of Nup358 (green) in HeLa cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 1% Blocker BSA in PBS (Product # 37525), each for 15 minutes at room temperature. Cells were stained with a Nup358 monoclonal antibody (Product # MA1-847) at a concentration of 10 µg/mL in 1% Blocker BSA in PBS, followed by a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were... View More
产品信息
MA1-847
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
2E1
抗原
Recombinant protein corresponding to 150 N-terminal residues of human Nup358.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2176789
产品详细信息
MA1-847 detects Nup358/RanBP2 from human samples.
靶标信息
RanBP2 interacts with the small GTP-binding protein Ran and forms a large multiprotein complex involved in many biological processes such as mRNA processing, nuclear transport, cell cycle control and postmitotic nuclear reassembly. The protein is identified by western blotting as an apparent molecular mass of 360 kD. It is involved in nucleation of microtubule and control of cell growth by interacting with many protein factors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 358 kDa nucleoporin; acute necrotizing encephalopathy 1 (autosomal dominant); E3 SUMO-protein ligase RanBP2; Nuclear pore complex protein Nup358; nucleoporin 358; Nucleoporin Nup358; P270; Ran-binding protein 2; RanBP2; RBP2; transformation-related protein 2
基因别名: ADANE; ANE1; IIAE3; NUP358; RANBP2; TRP1; TRP2
UniProt ID: (Human) P49792
Entrez Gene ID: (Human) 5903
protein peptidyl-prolyl isomerization
mRNA export from nucleus
tRNA export from nucleus
protein folding
NLS-bearing protein import into nucleus
sister chromatid cohesion
mitotic nuclear envelope disassembly
regulation of glucose transport
viral process
protein sumoylation
viral transcription
gene silencing by RNA
negative regulation of glucokinase activity
intracellular transport of virus
regulation of gluconeogenesis involved in cellular glucose homeostasis
regulation of cellular response to heat
