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RAP1GAP Antibody (PA5-78625) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of RAP1GAP was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RAP1GAP Polyclonal Antibody (Product # PA5-78625) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
产品信息
PA5-78625
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the C-terminus region of mouse Rap1GAP. The exact sequence is proprietary.
偶联物
Unconjugated
形式
Liquid
浓度
0.3 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2736497
产品详细信息
Positive Control: mouse brain, rat brain
Predicted Reactivity: Human (81%), Bovine (80%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and also attenuates the ability of activated G alpha z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ARPP-90; Rap1 GTPase-activating protein 1; Rap1, GTPase-activating protein 1; Rap1GAP
基因别名: 1300019I11Rik; 2310004O14Rik; AI427470; ARPP-90; Kiaa0474; Rap1ga1; Rap1gap; Rap1GAP1
UniProt ID: (Mouse) A2ALS5
Entrez Gene ID: (Mouse) 110351, (Rat) 313644
