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RASSF1A Antibody (14-6888-82) in ICC/IF
The Biochemical journal 2010 -
Figure 3 Analysis of mitotic signalling proteins in RASSF7 -knockdown HeLa cells ( A ) Active PLK1 (green) appeared normal. ( B ) RASSF1A (green) appeared normal. ( C and D ) Aurora A (red, arrows) and Aurora B (red, arrowheads) appeared to localize normally. Phospho-Aurora at the centrosomes (green, arrows) appeared normal, but phospho-Aurora at the kinetochore (green, arrowheads) was reduced (9.3% of cell... View More
产品信息
14-6888-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
eB114-10H1
偶联物
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_468373
产品详细信息
Description: The eB114 antibody reacts with the RASSF1A isoform of the tumor suppressor gene RASSF1. RASSF1A contains a Ras Association Domain (RA) and binds Ras in a GTP-dependent manner to effect apoptosis. The RASSF1A isoform undergoes epigenetic inactivation in a variety of human primary tumors. This has been associated with hypermethylation of a CpG island(s) in its promoter region. This antibody was generated against a synthetic peptide corresponding to the splice junction region of RASSF1A.
Applications Reported: The eB114 antibody has been reported for use in immunoprecipitation, and immunoblotting (WB).
Applications Tested: The eB114-10H1 has been tested by Western blotting and can be used at approximately 1-10 µg/mL. RASS1A is detected as a 45 kDa band in Western blotting of a HeLa cell extract. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cardiac-specific ras association domain family 1 protein; pancreas-specific ras association domain family 1 protein; Ras association (RalGDS/AF-6) domain family member 1; Ras association domain-containing protein 1; RASSF1; tumor suppressor protein RDA32; WUGSC:H_LUCA12.5
基因别名: 123F2; NORE2A; RASSF1; RASSF1A; RDA32; REH3P21
UniProt ID: (Human) Q9NS23
Entrez Gene ID: (Human) 11186
