Invitrogen

RGN Polyclonal Antibody

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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RGN Antibody in Immunohistochemistry (IHC)

RGN Antibody in Immunohistochemistry (IHC)

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RGN Antibody (PA5-56057) in IHC

Immunohistochemical staining of RGN in human adrenal gland, kidney, liver and testis using RGN Polyclonal Antibody (Product # PA5-56057) (A) shows similar protein distribution across tissues to an independent RGN Polyclonal Antibody (B).

RGN Antibody in Immunohistochemistry (IHC)

RGN Antibody in Immunohistochemistry (IHC)

RGN Antibody in Immunohistochemistry (IHC)

RGN Antibody in Immunohistochemistry (IHC)

RGN Antibody in Immunohistochemistry (IHC)

RGN Antibody in Immunohistochemistry (IHC)

RGN Antibody in Western Blot (WB)

RGN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

RGN Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of RGN in human adrenal gland, kidney, liver and testis using RGN Polyclonal Antibody (Product # PA5-56057) (A) shows similar protein distribution across tissues to an independent RGN Polyclonal Antibody (B).

RGN Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of RGN in adrenal gland using a RGN Polyclonal Antibody (Product # PA5-56057) shows strong cytoplasmic, nuclear and membranous positivity in cortical cells.

RGN Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of RGN in human adrenal gland using RGN Polyclonal Antibody (Product # PA5-56057) shows high expression.

RGN Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of RGN in human cerebral cortex using RGN Polyclonal Antibody (Product # PA5-56057) shows low expression as expected.

RGN Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of RGN in human kidney using RGN Polyclonal Antibody (Product # PA5-56057).

RGN Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of RGN in human testis using RGN Polyclonal Antibody (Product # PA5-56057).

RGN Antibody in Western Blot (WB) — Western blot analysis of RGN using RGN Polyclonal Antibody (Product # PA5-56057) (A) shows similar pattern to an independent RGN Polyclonal Antibody (B).

RGN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Identification of RGN expression in LUSC from TCGA, GEO databases and clinical specimens. ( A ) RGN expression between normal and tumor tissues in pan-cancer from TCGA database. ( B ) Expression levels of RGN mRNA in pan-cancer. ( C , D ) Representative immunohistochemistry images of RGN in LUSC and adjacent non-tumor tissues. The expression level of RGN in tumor tissues was significantly lower than that in adjacent non-tumor tissues. ( E ) RGN mRNA expression in tumor adjacent non-tumor tissues. ( F ) The level of RGN expression was lower in LUSC compared with normal tissues in different datasets. ( G ) Diagnostic value of RGN expression in LUSC in GSE14228 dataset and TCGA database. ( H ) Paired analysis of tumor and normal tissues based on RGN expression in LUSC in GSE19188 and GSE33532 datasets. * P < 0.05; ** P < 0.01; *** P < 0.001.

RGN Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of RGN demonstrates antibody specificity. Immunohistochemical analysis of RGN using anti-RGN Polyclonal Antibody (Product # PA5-56057), shows significant staining of RGN in human adrenal gland and shows minimal or weak staining in human cerebral cortex tissues. The relative expression levels of RGN within each tissue is shown using RNA-Seq. {RE}

产品信息

PA5-56057

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

-

免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:500-1:1,000

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human RGN. Recombinant protein control fragment (Product #RP-95633).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2646511

产品详细信息

Immunogen sequence: DAEGKLWVAC YNGGRVIRLD PVTGKRLQTV KLPVDKTTSC CFGGKNYSEM YVTCARDGMD PEGLLRQPEA GGIFKITGLG VKGIAPYSY

Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 92%.

靶标信息

The protein encoded by this gene is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. It may have an important role in calcium homeostasis. Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation. This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23. Alternative splicing results in two transcript variants having different 5' UTRs, but encoding the same protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

RGN as a prognostic biomarker with immune infiltration and ceRNA in lung squamous cell carcinoma.

Scientific reports

Liao Y,Cheng W,Mou R,Li X,Jia Y

Published figure using RGN polyclonal antibody (Product # PA5-56057) in Immunohistochemistry (Paraffin)

Tue May 09 00:00:00 EDT 2023

RGN as a prognostic biomarker with immune infiltration and ceRNA in lung squamous cell carcinoma.

Scientific reports

Liao Y,Cheng W,Mou R,Li X,Jia Y

Published figure using RGN polyclonal antibody (Product # PA5-56057) in Immunohistochemistry (Paraffin)

Tue May 09 00:00:00 EDT 2023

生物信息学

蛋白别名: epididymis secretory protein Li 41; Gluconolactonase; GNL; RC; Regucalcin; Senescence marker protein 30; SMP-30

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基因别名: AI265316; GNL; HEL-S-41; RC; Reguc; RGN; SMP30

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UniProt ID: (Human) Q15493, (Rat) Q03336, (Mouse) Q64374

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Entrez Gene ID: (Human) 9104, (Rat) 25106, (Mouse) 19733

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gluconolactonase activity calcium ion binding zinc ion binding enzyme regulator activity hydrolase activity metal ion binding esterase

参与通路

kidney development negative regulation of protein kinase activity cellular calcium ion homeostasis spermatogenesis aging positive regulation of triglyceride biosynthetic process positive regulation of glucose metabolic process positive regulation of phosphatase activity L-ascorbic acid biosynthetic process negative regulation of phosphoprotein phosphatase activity positive regulation of ATPase activity negative regulation of GTPase activity negative regulation of apoptotic process positive regulation of GTPase activity negative regulation of nitric oxide biosynthetic process positive regulation of fatty acid biosynthetic process negative regulation of epithelial cell proliferation regulation of calcium-mediated signaling negative regulation of cyclic-nucleotide phosphodiesterase activity liver regeneration negative regulation of sperm motility positive regulation of superoxide dismutase activity positive regulation of calcium-transporting ATPase activity negative regulation of RNA biosynthetic process negative regulation of bone development positive regulation of proteolysis involved in cellular protein catabolic process negative regulation of calcium-dependent ATPase activity negative regulation of DNA catabolic process positive regulation of dUTP diphosphatase activity negative regulation of leucine-tRNA ligase activity negative regulation of DNA biosynthetic process liver development negative regulation of protein phosphorylation