Invitrogen

RGS14 Polyclonal Antibody

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RGS14 Antibody (PA5-18278) in WB

Western blot analysis of RGS14 using RGS14 Polyclonal Antibody (Product # PA5-18278) (0.5 µg/mL) in staining of Jurkat lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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产品信息

PA5-18278

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (IGGSLNSTTDSAL) corresponding to the C-terminus amino acids of RGS14 (aa 554-566).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_10979893

产品详细信息

This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

靶标信息

RGS14 is a member of the regulator of G-protein signaling family which contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. RGS14 attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. The resulting hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of RGS14 have been observed but have not been thoroughly characterized. Further, RGS14 acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism; plays a role in cell division; is required for the nerve growth factor (NGF)-mediated neurite outgrowth, and is involved in stress resistance. RGS14 protein may also be involved in visual memory processing capacity and hippocampal-based learning and memory.

仅用于科研。不用于诊断过程。未经明确授权不得转售。