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Invitrogen

RHEB Monoclonal Antibody (GT39810)

高级验证

查看其他13个RHEB抗体

说明书

实验方案

问题与答案

技术支持

货号 MA5-27777

CNY 6,133.00

100 µL

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说明书

实验方案

问题与答案

技术支持

抗体检测数据 (7)
高级验证 (1)

Application

RHEB Antibody in Immunocytochemistry (ICC/IF)

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RHEB Antibody (MA5-27777) in ICC/IF

Immunofluorescence analysis of RHEB was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RHEB Monoclonal Antibody (GT39810) (Product # MA5-27777) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Produ... View More

RHEB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RHEB Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RHEB siRNA and loss of signal intensity was observed in Western Blot application using Anti-RHEB Monoclonal Antibody (GT39810) (Product # MA5-27777). {KD}

产品信息

MA5-27777

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

GT39810

抗原

Recombinant protein encompassing a sequence within the center region of human Rheb. The exact sequence is proprietary.

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2735346

产品详细信息

Positive Control: SK-N-SH

Predicted Reactivity: Mouse (98%), Rat (99%), Zebrafish (90%), Xenopus laevis (91%), Pig (99%), Sheep (99%), Rhesus Monkey (100%), Bovine (98%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Rheb encodes a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.

仅用于科研。不用于诊断过程。未经明确授权不得转售。