Invitrogen

RIP3 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4个已发表图片

4 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

RIP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 11

RIP3 Antibody (PA5-19956) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using RIP3 Polyclonal Antibody (Product # PA5-19956) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.... View More

RIP3 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

RIP3 Antibody — Supplemental Figure 2 Treatment with CBDCA and anti-PD-1 antibodies increases levels of necroptosis in the tumor. Necroptotic cells in the implanted cancer were determined by RIP3 staining by immunofluorescence at day 24 after the initial implantation. (A) Representative images of RIP3 staining. scale bar: 43 mum. Treatment of CBDCA and alpha-PD-1 antibodies increased levels of necroptosis in 4T1 (B) , EMT6 (C) , and E0771 (D) tumors. n = 10 per group. **** p < 0.0001, by one-way ANOVA with Bonferroni post-hoc tests. Click here for additional data file. {TM}

产品信息

PA5-19956

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

- -

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

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免疫沉淀 (IP)

20 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A 14 amino acid peptide near the carboxy terminus of murine RIP3

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11154832

产品详细信息

A suggested positive control is NIH-3T3 cell lysate.

PA5-19956 can be used with blocking peptide PEP-0081.

The PA5-19956 immunogen is located within the last 50 amino acids of RIP3.

Predicted molecular ~ 53kD for mouse RIP3 and 57kD for human RIP3. In Western blot applications, this antibody has been observed to detect a band at: 53kD for mouse RIP3 and 57kD for human RIP3

靶标信息

RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, which contains a C-terminal domain unique from other RIP family members. RIPK3 is predominantly localized to the cytoplasm, and specifically, are colocalized in the mitochondrion which can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. RIPK3 is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor and caspase-8 prevents RIPK3-dependent necrosis without inducing apoptosis by functioning in a proteolytically active complex with CFLAR and that this complex is required for the protective function.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Acetovanillone augmented the cardioprotective effect of carvedilol against cadmium-induced heart injury via suppression of oxidative stress and inflammation signaling pathways.

Scientific reports

Hassanein EHM,Bakr AG,El-Shoura EAM,Ahmed LK,Ali FEM

PA5-19956 was used in Western Blotting to investigate the effectiveness of carvedilol (CV), Acetovanillone (ACET), and their combination for ameliorating cadmium (Cd)-induced oxidative stress, inflammation, and necroptosis.

Fri Mar 31 00:00:00 EDT 2023

Acetovanillone augmented the cardioprotective effect of carvedilol against cadmium-induced heart injury via suppression of oxidative stress and inflammation signaling pathways.

Scientific reports

Hassanein EHM,Bakr AG,El-Shoura EAM,Ahmed LK,Ali FEM

Fri Mar 31 00:00:00 EDT 2023

Symbiotic microbiome Staphylococcus epidermidis restricts IL-33 production in allergic nasal epithelium via limiting the cellular necroptosis.

BMC microbiology

Jeon YJ,Gil CH,Won J,Jo A,Kim HJ

Published figure using RIP3 polyclonal antibody (Product # PA5-19956) in Western Blot

Fri May 26 00:00:00 EDT 2023

Therapy With Carboplatin and Anti-PD-1 Antibodies Before Surgery Demonstrates Sustainable Anti-Tumor Effects for Secondary Cancers in Mice With Triple-Negative Breast Cancer.

Frontiers in immunology

Gao M,Wang T,Ji L,Bai S,Tian L,Song H

Published figure using RIP3 polyclonal antibody (Product # PA5-19956) in Immunocytochemistry

Mon Mar 01 00:00:00 EST 2021

Autophosphorylation at serine 166 regulates RIP kinase 1-mediated cell death and inflammation.

Nature communications

Laurien L,Nagata M,Schünke H,Delanghe T,Wiederstein JL,Kumari S,Schwarzer R,Corona T,Krüger M,Bertrand MJM,Kondylis V,Pasparakis M

PA5-19956 was used in Western Blotting to show that S166 autophosphorylation licenses RIPK1 kinase activity to induce downstream cell death signaling and inflammation, suggesting that S166 phosphorylation can serve as a reliable biomarker for RIPK1 kinase-dependent pathologies.

Wed Apr 08 00:00:00 EDT 2020

Autophosphorylation at serine 166 regulates RIP kinase 1-mediated cell death and inflammation.

Nature communications

Laurien L,Nagata M,Schünke H,Delanghe T,Wiederstein JL,Kumari S,Schwarzer R,Corona T,Krüger M,Bertrand MJM,Kondylis V,Pasparakis M

Wed Apr 08 00:00:00 EDT 2020

生物信息学

蛋白别名: Homocysteine respondent protein HCYP2; mRIP3; receptor interacting protein 3; Receptor-interacting protein 3; receptor-interacting serine-threonine kinase 3; Receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3

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基因别名: 2610528K09Rik; AW107945; Hcyp2; RIP3; RIPK3

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UniProt ID: (Human) Q9Y572, (Mouse) Q9QZL0, (Rat) Q9Z2P5

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Entrez Gene ID: (Human) 11035, (Mouse) 56532, (Rat) 246240

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