Invitrogen

RIP3 Recombinant Rabbit Monoclonal Antibody (21H67L3)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

查看其他25个RIP3抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

图: 1 / 3

RIP3 Antibody (702660) in WB

Western blot analysis was performed on membrane extracts (30 µg lysate) of HT-29 (Lane 1), Ramos (Lane 2) Raji (Lane 3) and K-562 (Lane 4). The blots were probed with Anti-RIP3 Recombinant Rabbit Monoclonal Antibody (Product # 702660, 1 µg/mL). A 57 kDa band corresponding to RIP3 was observed across the cell lines tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:5000 dilution). Known quantity of protein samples were e... View More

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RIP3 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of RIP3 was observed in HT-29 and HCT 116 using Anti-RIP3 Recombinant Rabbit Monoclonal Antibody (Product # 702660) in Western blot. HT-29 and HCT-116 (colorectal carcinoma) are documented to have differential basal expression of RIP-3, where-in RIP3 expression is higher in HT-29 when compared to HCT-116. {RE}

产品信息

702660

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

21H67L3

抗原

Peptide corresponding to human RIP3 [aa496-aa513]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2689473

产品详细信息

This antibody is predicted to react with Mouse, Rat, Horse

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, which contains a C-terminal domain unique from other RIP family members. RIPK3 is predominantly localized to the cytoplasm, and specifically, are colocalized in the mitochondrion which can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. RIPK3 is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor and caspase-8 prevents RIPK3-dependent necrosis without inducing apoptosis by functioning in a proteolytically active complex with CFLAR and that this complex is required for the protective function.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: receptor interacting protein 3; Receptor-interacting protein 3; receptor-interacting serine-threonine kinase 3; Receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3

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基因别名: RIP3; RIPK3

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UniProt ID: (Human) Q9Y572

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Entrez Gene ID: (Human) 11035

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功能

transcription coactivator activity protein kinase activity protein serine/threonine kinase activity NF-kappaB-inducing kinase activity protein binding ATP binding protein complex binding identical protein binding non-receptor serine/threonine protein kinase

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