Invitrogen

ROCK1 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

4个已发表图片

5 篇参考文献

查看其他24个ROCK1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

ROCK1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

ROCK1 Antibody (PA5-22262) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of ROCK1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ROCK1 Polyclonal Antibody (Product # PA5-22262) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

ROCK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ROCK1 Antibody in Immunoprecipitation (IP)

ROCK1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

ROCK1 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in ROCK1 knockout (KO) Hela cell line using Anti-ROCK1 Polyclonal Antibody (Product # PA5-22262). {KO}

产品信息

PA5-22262

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 107 and 371 (Q13464) of ROCK1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.35 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_11155392

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, C2Cl2, rat brain.

Predicted reactivity: Human (98%), Mouse (98%), Rat (98%), Zebrafish (90%), Rabbit (98%), Chicken (96%), Rhesus Monkey (98%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model.

Nature communications

Hu M,Li T,Ma X,Liu S,Li C,Huang Z,Lin Y,Wu R,Wang S,Lu D,Lu T,Men X,Shen S,Huang H,Liu Y,Song K,Jian B,Jiang Y,Qiu W,Liu Q,Lu Z,Cai W

PA5-22262 was used in Western Blotting to propose that macrophage-derived migrasomes and the consequent complement activation are potential biomarkers and therapeutic targets in cerebral amyloid angiopathy (CAA).

Tue Jul 04 00:00:00 EDT 2023

Endothelial cell-derived SSAO can increase MLC₂₀ phosphorylation in VSMCs.

Open life sciences

Zhang Y,Zhang X,Cao Z,Huang Y,Zheng Y,Yang X

PA5-22262 was used in Western Blotting to find that the increased SSAO by AOC3 overexpression can decrease the iNOS expression level and its activity after hypoxia.

Tue Nov 02 00:00:00 EDT 2021

Endothelial cell-derived SSAO can increase MLC₂₀ phosphorylation in VSMCs.

Open life sciences

Zhang Y,Zhang X,Cao Z,Huang Y,Zheng Y,Yang X

PA5-22262 was used in Western Blotting to find that the increased SSAO by AOC3 overexpression can decrease the iNOS expression level and its activity after hypoxia.

Tue Nov 02 00:00:00 EDT 2021

Circ_0057558 promotes nonalcoholic fatty liver disease by regulating ROCK1/AMPK signaling through targeting miR-206.

Cell death & disease

Chen X,Tan QQ,Tan XR,Li SJ,Zhang XX

Published figure using ROCK1 polyclonal antibody (Product # PA5-22262) in Immunohistochemistry

Thu Aug 26 00:00:00 EDT 2021

Periodic propagating waves coordinate RhoGTPase network dynamics at the leading and trailing edges during cell migration.

eLife

Bolado-Carrancio A,Rukhlenko OS,Nikonova E,Tsyganov MA,Wheeler A,Garcia-Munoz A,Kolch W,von Kriegsheim A,Kholodenko BN

PA5-22262 was used in Immunocytochemistry to elaborate the minimal autonomous biochemical machinery necessary and sufficient for dynamic trailing edge coordination and cell movement.

Fri Jul 24 00:00:00 EDT 2020

Periodic propagating waves coordinate RhoGTPase network dynamics at the leading and trailing edges during cell migration.

eLife

Bolado-Carrancio A,Rukhlenko OS,Nikonova E,Tsyganov MA,Wheeler A,Garcia-Munoz A,Kolch W,von Kriegsheim A,Kholodenko BN

PA5-22262 was used in Immunocytochemistry to elaborate the minimal autonomous biochemical machinery necessary and sufficient for dynamic trailing edge coordination and cell movement.

Fri Jul 24 00:00:00 EDT 2020

Rho-associated kinase 1 inhibition is synthetically lethal with von Hippel-Lindau deficiency in clear cell renal cell carcinoma.

Oncogene

Thompson JM,Nguyen QH,Singh M,Pavesic MW,Nesterenko I,Nelson LJ,Liao AC,Razorenova OV

Published figure using ROCK1 polyclonal antibody (Product # PA5-22262) in Western Blot

Thu Feb 23 00:00:00 EST 2017

Rho-associated kinase 1 inhibition is synthetically lethal with von Hippel-Lindau deficiency in clear cell renal cell carcinoma.

Oncogene

Thompson JM,Nguyen QH,Singh M,Pavesic MW,Nesterenko I,Nelson LJ,Liao AC,Razorenova OV

Published figure using ROCK1 polyclonal antibody (Product # PA5-22262) in Western Blot

Thu Feb 23 00:00:00 EST 2017

生物信息学

蛋白别名: liver regeneration-related protein LRRG199; MGC131603; MGC43611; p150 RhoA-binding kinase ROK beta; p160 ROCK-1; p160-ROCK; p160ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; Rho-associated coiled-coil forming kinase 1; Rho-associated kinase beta; Rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I

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基因别名: 1110055K06Rik; P160ROCK; ROCK-I; ROCK1

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UniProt ID: (Human) Q13464, (Mouse) P70335

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Entrez Gene ID: (Human) 6093, (Mouse) 19877, (Rat) 81762

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功能