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Bethyl Laboratories
ROCK2 Polyclonal Antibody
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FIGURE: 1 / 2
ROCK2 Antibody (A300-047A) in WB
Detection of human ROCK2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROCK2 antibody (Product # A300-047A) (lot A300-047A-1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A300-047A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residues 1320 and the C-terminus (residues 1388) of Human ROCK2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat and Bovine
Target Information
ROCK2, the predominant ROCK isoform in skeletal muscle, is an important regulator downstream of Rho GTPases. ROCK2 regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: p164 ROCK-2; Rho kinase 2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK II; ROCK-II; rockii
Gene Aliases: KIAA0619; ROCK-II; ROCK2
UniProt ID: (Human) O75116
Entrez Gene ID: (Human) 9475
cytokinesis
positive regulation of protein phosphorylation
protein phosphorylation
smooth muscle contraction
I-kappaB kinase/NF-kappaB signaling
Rho protein signal transduction
positive regulation of endothelial cell migration
positive regulation of gene expression
positive regulation of centrosome duplication
negative regulation of angiogenesis
regulation of cell adhesion
cortical actin cytoskeleton organization
regulation of actin cytoskeleton organization
negative regulation of myosin-light-chain-phosphatase activity
regulation of circadian rhythm
regulation of keratinocyte differentiation
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
rhythmic process
centrosome duplication
regulation of stress fiber assembly
regulation of focal adhesion assembly
cellular response to testosterone stimulus
establishment of protein localization to plasma membrane
regulation of establishment of endothelial barrier
negative regulation of bicellular tight junction assembly
regulation of establishment of cell polarity
regulation of cell motility
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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