Invitrogen

ROCK2 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2个已发表图片

1 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

ROCK2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

ROCK2 Antibody (PA5-78290) in ICC/IF

Immunofluorescence analysis of ROCK2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ROCK2 Rabbit Polyclonal Antibody (Product # PA5-78290) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilut... View More

ROCK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ROCK2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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ROCK2 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of target protein. 293T cells were transfected with ROCK2 shRNA and decrease in signal intensity was observed in Western blotting using Anti-ROCK2 Polyclonal Antibody (Product # PA5-78290). {KD}

产品信息

PA5-78290

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.4 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2736534

产品详细信息

Positive Control: 293T, A431, HeLa, HepG2, Rat2, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, A549, H1299, HCT116

Predicted Reactivity: Xenopus laevis (80%), Rhesus Monkey (100%), Bovine (97%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ROCK2, the predominant ROCK isoform in skeletal muscle, is an important regulator downstream of Rho GTPases. ROCK2 regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Lamin A and the LINC complex act as potential tumor suppressors in Ewing Sarcoma.

Cell death & disease

Chiarini F,Paganelli F,Balestra T,Capanni C,Fazio A,Manara MC,Landuzzi L,Petrini S,Evangelisti C,Lollini PL,Martelli AM,Lattanzi G,Scotlandi K

Published figure using ROCK2 polyclonal antibody (Product # PA5-78290) in Western Blot

Thu Apr 14 00:00:00 EDT 2022

生物信息学

蛋白别名: p150 ROK-alpha; p164 ROCK-2; Rho kinase 2; Rho-associated coiled-coil forming kinage 2; Rho-associated coiled-coil forming kinase 2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; RhoA - binding serine/threosine kinase alpha (ROK - alpha); RhoA-binding kinase 2; ROCK II; ROCK-II; rockii; ROKalpha

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基因别名: B230113H15Rik; KIAA0619; mKIAA0619; Rho-kinase; ROCK-II; ROCK2; Rock2m; ROK; ROKalpha

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UniProt ID: (Human) O75116, (Rat) Q62868, (Mouse) P70336

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Entrez Gene ID: (Human) 9475, (Rat) 25537, (Mouse) 19878

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