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RPA70 Antibody (MA5-35367) in WB
Western blot analysis of RPA70 in extracts of various cell lines. Samples were incubated with RPA70 Monoclonal antibody (Product # MA5-35367) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
MA5-35367
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
ARC0773
抗原
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPA70/RPA1 (P27694)
偶联物
形式
Liquid
浓度
0.55 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2849268
产品详细信息
Immunogen sequence: MVGQLSEGAI AAIMQKGDTN IKPILQVINI RPITTGNSPP RYRLLMSDGL NTLSSFMLAT QLNPLVEEEQ LSSNCVCQIH RFIVNTLKDG RRVVILMELE
靶标信息
This gene Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: MSTP075; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; replication protein A1, 70kDa; RF-A protein 1; RP-A p70; Single-stranded DNA-binding protein
基因别名: Cb1-727; HSSB; MST075; REPA1; RF-A; RP-A; RPA1; RPA70
UniProt ID: (Human) P27694
Entrez Gene ID: (Human) 6117, (Rat) 287524
G1/S transition of mitotic cell cycle
telomere maintenance via recombination
telomere maintenance
double-strand break repair via homologous recombination
DNA replication
DNA-dependent DNA replication
DNA repair
transcription-coupled nucleotide-excision repair
base-excision repair
nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
mismatch repair
DNA recombination
protein sumoylation
translesion synthesis
nucleotide-excision repair, DNA incision
interstrand cross-link repair
error-prone translesion synthesis
DNA damage response, detection of DNA damage
error-free translesion synthesis
regulation of cellular response to heat
regulation of signal transduction by p53 class mediator
in utero embryonic development
meiotic nuclear division
positive regulation of cell proliferation
hemopoiesis
homeostasis of number of cells within a tissue
chromosome organization
