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Invitrogen
F(ab)-Rabbit anti-Goat IgG Fc Secondary Antibody, Alexa Fluor™ 647
高级验证
This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.
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Goat IgG Fc Secondary Antibody (A66794) in ICC/IF
Immunofluorescence analysis of F(ab)-Rabbit anti-Goat IgG Fc Secondary Antibody, Alexa Fluor™ 647 (Product # A66794) was performed on fixed and permeabilized HeLa cells blocked with 2% BSA at room temperature and stained with HSPA9 Polyclonal Antibody (Product # PA5-48035, 0.4 µg/mL) in 0.1% BSA overnight at 4-degree Celsius. This was detected by incubating with F(ab)-Rabbit anti-Goat IgG Fc Secondary Antib... View More
产品信息
A66794
应用
建议稀释比
免疫印迹 (WB)
0.4-0.5 µg/mL
免疫组化（石蜡） (IHC (P))
1 µg/mL
免疫组织化学（多聚甲醛固定） (IHC (PFA))
1 µg/mL
免疫细胞化学 (ICC/IF)
0.5-2 µg/mL
流式细胞分析 (Flow)
1 µg/mL
肽阵列 (Array)
Not Cited
产品规格
种属反应
Goat
宿主/亚型
Rabbit
/ IgG, F(ab)
分类
Polyclonal
类型
Secondary Antibody
抗原
Goat IgG Fc
偶联物
激发/发射光谱
650/671 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Size-exclusion chromatography
保存液
PBS, pH 7.5
内含物
5mM sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient
靶标
IgG Fc
抗体形式
F(ab) Fragment
产品详细信息
This F(ab)-Rabbit anti-Goat IgG Fc specific antibody product is a rabbit polyclonal antibody F(ab) fragment that is specific to the Fc region of goat IgG. The product has minimal to no cross-reactivity towards polyclonal mouse, rat, guinea pig, bovine, hamster, horse, sheep, human, monkey, donkey IgG, and chicken IgY as tested by direct ELISA. Cross-reactivity towards other immunoglobulins has not been tested. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
F(ab) antibody fragment offer several benefits over whole IgGs, due to their smaller size (~50 kD vs. ~150 kD) and the absence of the Fc region. The smaller size is expected to allow better penetration into tissue sections and improved staining in applications such as IHC. The lack of Fc region results in reduced nonspecific background staining when staining cells/ tissues with Fc receptors (such as immune cells) and other Fc interaction-related interference. Fc specific F(ab) fragments provide a range of multiplexing options beyond what is possible with whole IgGs for applications such as immunocytochemistry, immunohistochemistry, flow cytometry, and western blotting.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
靶标信息
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
