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Invitrogen
Rabbit anti-Human IgG Fc gamma Secondary Antibody
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Human IgG Fc gamma Secondary Antibody (31142) in ICC/IF
Immunofluorescence analysis of Rabbit anti-Human IgG Fc gamma Secondary Antibody was performedusing log phaseIM9cells (IgG producing B-cell line). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled withRabbit anti-Human IgG Fc gamma Secondary Antibody (Product # 31142... View More
产品信息
31142
应用
建议稀释比
免疫印迹 (WB)
1:6000
免疫组化 (IHC)
Assay-dependent
免疫细胞化学 (ICC/IF)
1:100
流式细胞分析 (Flow)
Assay-dependent
免疫沉淀 (IP)
Assay-dependent
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
形式
Liquid
浓度
2.3 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.6
内含物
no preservative
保存条件
4° C
运输条件
Ambient
RRID
AB_228405
靶标
IgG Fc
抗体形式
Whole Antibody
产品详细信息
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31142 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product #31142 reacts with the heavy chains of human IgG, but not with the light chains to most human immunoglobulins or with non-immunoglobulin serum proteins. This antibody does not react with human IgM or IgA. However, this antibody may cross-react with immunoglobulins from other species.
Store product at 4°C until opened. After opening, dilute only enough antibody for a single day's use. Store remainder at 4°C under sterile conditions.
Country of Origin: USA
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
