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Rat IgG (H+L) Secondary Antibody (PA1-28570) in ICC/IF
Immunofluorescence analysis of Rabbit anti-Rat IgG Secondary Antibody, TRITC was performed using A549 cells stained with alpha Tubulin (YL1/2) Rat Monoclonal Antibody (Product # MA1-80017). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2µg/mL Rat primary antibody for 3 hours at room temperat... View More
产品信息
PA1-28570
应用
建议稀释比
免疫细胞化学 (ICC/IF)
1:1,000-1:5,000
流式细胞分析 (Flow)
1:500-1:2500
产品规格
种属反应
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Rat IgG whole molecule
偶联物
激发/发射光谱
552/578 nm
查看光谱
形式
Liquid
浓度
2 mg/mL
纯化类型
IgG fraction
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA
内含物
0.01% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10987094
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
