Bethyl Laboratories

Rad50 Polyclonal Antibody

查看其他20个RAD50抗体

抗体检测数据 (2)

Rad50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Rad50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Rad50 Antibody (A300-185A) in IHC (P)

Detection of human Rad50 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Rad50 (Product # A300-185A) (Lot 1) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

Rad50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Rad50 Antibody in Western Blot (WB)

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Rad50 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Detection of human Rad50 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Rad50 (Product # A300-185A) (Lot 1) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

Rad50 Antibody in Western Blot (WB) — Detection of human Rad50 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad50 antibody (Product # A300-185A) (lot A300-185A-1) used for WB at 0.2 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

产品信息

A300-185A

应用

建议稀释比

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免疫印迹 (WB)

1:1,000-1:5,000

-

免疫组化 (IHC)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:500-1:2,000

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residues 700 and 750 of Human Rad50.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

phosphate/tris citrate, pH 7-8

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Bovine, Dog, Rabbit, Orangutan, Monkey, Gorilla and Chimpanzee

靶标信息

Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DNA repair protein RAD50; hRAD50; hsRAD50; RAD50 2; RAD50 homolog, double strand break repair protein; RAD50 PEN; RAD50-2; RAD50-3

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基因别名: hRad50; NBSLD; RAD50; RAD502

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UniProt ID: (Human) Q92878

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Entrez Gene ID: (Human) 10111

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single-stranded DNA endodeoxyribonuclease activity DNA binding double-stranded telomeric DNA binding ATP-dependent DNA helicase activity adenylate kinase activity protein binding ATP binding 3'-5' exonuclease activity protein binding, bridging single-stranded telomeric DNA binding metal ion binding G-quadruplex DNA binding

参与通路

regulation of mitotic recombination telomere maintenance via recombination telomere maintenance double-strand break repair via homologous recombination DNA double-strand break processing DNA synthesis involved in DNA repair strand displacement DNA replication DNA repair double-strand break repair double-strand break repair via nonhomologous end joining DNA recombination cellular response to DNA damage stimulus telomere maintenance via telomerase reciprocal meiotic recombination viral process telomeric 3' overhang formation positive regulation of protein autophosphorylation positive regulation of telomere maintenance DNA duplex unwinding positive regulation of kinase activity nucleotide phosphorylation chromosome organization involved in meiotic cell cycle nucleic acid phosphodiester bond hydrolysis regulation of signal transduction by p53 class mediator negative regulation of telomere capping