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Invitrogen
Rat IgG1 kappa Isotype Control (eBRG1), APC-eFluor™ 780, eBioscience™
产品信息
47-4301-80
应用
建议稀释比
流式细胞分析 (Flow)
Assay-Dependent
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Rat
/ IgG1, kappa
分类
Monoclonal
类型
Isotype Control
克隆号
eBRG1
偶联物
激发/发射光谱
756/785 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1271986
产品详细信息
Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.
Applications Reported: APC-eFluor® 780 Rat IgG1 Isotype Control (APC-Alexa Fluor® 750 replacement) has been reported for use in flow cytometric analysis.
Applications Tested: Rat IgG1 K Isotype Control APC-eFluor® 780 has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody.
APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics. These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants. Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens. It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens. Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases. With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
